Inorganic Hg toxicity in plants: A comparison of different genotoxic parameters

Plant Physiology and Biochemistry : PPB
Raquel AzevedoConceição Santos

Abstract

Inorganic Mercury (Hg) contamination persists an environmental problem, but its cyto- and genotoxicity in plants remains yet unquantified. To determine the extent of Hg-induced cyto- and genotoxicity, and assess most sensitive endpoints in plants, Pisum sativum L. seedlings were exposed for 14 days to different HgCl2 concentrations up to 100 μM. Shoots and roots from hydroponic exposure presented growth impairment and/or morphological disorders for doses >1 μM, being the roots more sensitive. Plant growth, ploidy changes, clastogenicity (HPCV), cell cycle dynamics (G1-S-G2), Comet-tail moment (TM), Comet-TD, Mitotic-index (MI) and cell proliferation index (CPI) were used to evaluate Hg-induced cyto/genotoxicity. Both leaf and root DNA-ploidy levels, assessed by flow cytometry (FCM), remained unaltered after exposure. Root cell cycle impairment occurred at lower doses (≥1 μM) than structural DNA damages (≥10 μM). Cytostatic effects depended on the Hg concentration, with delays during S-phase at lower doses, and arrests at G1 at higher ones. This arrest was paralleled with decreases of both mitotic index (MI) and cell proliferation index (CPI). DNA fragmentation, assessed by the Comet assay parameters of TD and TM, could be visua...Continue Reading

Citations

Oct 13, 2019·Environmental Science and Pollution Research International·Eleazar RodriguezConceição Santos
Nov 5, 2019·Science Advances·Sofie LindströmPeter M Outridge
Feb 26, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Ting LiuChunming Liu

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