PMID: 9555903Apr 29, 1998Paper

Inorganic polyphosphate in Escherichia coli: the phosphate regulon and the stringent response

Journal of Bacteriology
N N RaoA Kornberg

Abstract

Escherichia coli transiently accumulates large amounts of inorganic polyphosphate (polyP), up to 20 mM in phosphate residues (Pi), in media deficient in both Pi and amino acids. This transient accumulation is preceded by the appearance of nucleotides ppGpp and pppGpp, generated in response to nutritional stresses. Mutants which lack PhoB, the response regulator of the phosphate regulon, do not accumulate polyP even though they develop wild-type levels of (p)ppGpp when subjected to amino acid starvation. When complemented with a phoB-containing plasmid, phoB mutants regain the ability to accumulate polyP. PolyP accumulation requires high levels of (p)ppGpp independent of whether they are generated by RelA (active during the stringent response) or SpoT (expressed during Pi starvation). Hence, accumulation of polyP requires a functional phoB gene and elevated levels of (p)ppGpp. A rapid assay of polyP depends on its adsorption to an anion-exchange disk on which it is hydrolyzed by a yeast exopolyphosphatase.

References

Jan 1, 1988·Annual Review of Biochemistry·H G Wood, J E Clark
Mar 27, 1973·Molecular & General Genetics : MGG·M Bracha, E Yagil
Sep 1, 1974·Journal of Bacteriology·F C NeidhardtD F Smith
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C W Hill, B W Harnish
Jul 1, 1984·Journal of Bacteriology·D LudtkeA Torriani
Jun 2, 1995·The Journal of Biological Chemistry·C E CastumaR N Reusch
Feb 1, 1995·Journal of Bacteriology·A Kornberg
Feb 1, 1995·Journal of Bacteriology·H WurstA Kornberg
Mar 17, 1995·The Journal of Biological Chemistry·K D Kumble, A Kornberg
Jun 4, 1993·Cell·J S Parkinson
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·T ShibaA Kornberg

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Citations

Jun 29, 2000·Annual Review of Biochemistry·A KornbergD Ault-Riché
Aug 31, 2000·Applied and Environmental Microbiology·J W McGrath, J P Quinn
Dec 2, 2000·Microbiology·M F AnjumJ R Guest
Apr 4, 2002·Molecular Microbiology·Hichem Chouayekh, Marie-Joelle Virolle
Oct 20, 2009·Journal of Bacteriology·Luciana Amado, Andrei Kuzminov
Oct 26, 2014·BMC Microbiology·Dalin RifatPetros C Karakousis
Feb 25, 2015·Applied and Environmental Microbiology·Luiz Gustavo de AlmeidaBeny Spira
Oct 17, 2016·Molecular Genetics and Genomics : MGG·Nicolás Federico Villamil MunévarBeny Spira
May 21, 2020·Frontiers in Microbiology·Bhaskar Chandra Mohan Ramisetty
Dec 30, 1999·Journal of Bacteriology·M H RashidA Kornberg
Aug 6, 2008·Annual Review of Genetics·Arthur BeauregardMarlene Belfort
Jan 15, 2008·Applied and Environmental Microbiology·Anne N ReidAlain Stintzi
Aug 19, 2008·Applied and Environmental Microbiology·Manfredo J SeufferheldMaria E Farias
Jan 1, 2011·Microbial Informatics and Experimentation·Sónia CarneiroIsabel Rocha
Oct 12, 2013·PloS One·Algevis P WrenchGraciela L Lorca
May 29, 2016·Journal of Bacteriology·Richard L Gourse, Sean Crosson
Sep 30, 2015·Antimicrobial Agents and Chemotherapy·Roberta ColicchioPaola Salvatore
Oct 6, 2000·Annual Review of Microbiology·I Kulaev, T Kulakovskaya
Jul 31, 2002·Applied and Environmental Microbiology·Tomohiro MorohoshiAkio Kuroda
Dec 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Haiyu ZhangArthur Kornberg
Feb 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Haiyu ZhangArthur Kornberg
Feb 4, 2010·The Journal of Biological Chemistry·Evgeny PavlovAndrey Y Abramov
Apr 7, 2009·Annual Review of Biochemistry·Narayana N RaoArthur Kornberg
Jul 3, 2016·Applied and Environmental Microbiology·Kelsey E VandenBergJonathan E Visick
Jan 11, 2016·World Journal of Microbiology & Biotechnology·Tomás Albi, Aurelio Serrano
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·A KurodaH Ohtake
Jul 12, 2001·Proceedings of the National Academy of Sciences of the United States of America·A SethuramanA Kornberg

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