Inorganic pyrophosphatase in uncultivable hemotrophic mycoplasmas: identification and properties of the enzyme from Mycoplasma suis.

BMC Microbiology
Katharina HoelzleLudwig E Hoelzle

Abstract

Mycoplasma suis belongs to a group of highly specialized hemotrophic bacteria that attach to the surface of host erythrocytes. Hemotrophic mycoplasmas are uncultivable and the genomes are not sequenced so far. Therefore, there is a need for the clarification of essential metabolic pathways which could be crucial barriers for the establishment of an in vitro cultivation system for these veterinary significant bacteria.Inorganic pyrophosphatases (PPase) are important enzymes that catalyze the hydrolysis of inorganic pyrophosphate PPi to inorganic phosphate Pi. PPases are essential and ubiquitous metal-dependent enzymes providing a thermodynamic pull for many biosynthetic reactions. Here, we describe the identification, recombinant production and characterization of the soluble (s)PPase of Mycoplasma suis. Screening of genomic M. suis libraries was used to identify a gene encoding the M. suis inorganic pyrophosphatase (sPPase). The M. suis sPPase consists of 164 amino acids with a molecular mass of 20 kDa. The highest identity of 63.7% was found to the M. penetrans sPPase. The typical 13 active site residues as well as the cation binding signature could be also identified in the M. suis sPPase. The activity of the M. suis enzyme w...Continue Reading

References

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Citations

Dec 10, 2013·Experimental Parasitology·Anita Leocadio Freitas-MesquitaJosé Roberto Meyer-Fernandes
Jul 21, 2016·Microbial Biotechnology·Antoine Danchin, Gang Fang
Feb 22, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ana M S GuimaraesJoanne B Messick
Sep 8, 2018·Journal of Proteome Research·Jatin ShrinetSujatha Sunil

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Datasets Mentioned

BETA
AE015450
FN394679

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

ClustalW
FASTA

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