iNOS-inhibitor driven neuroprotection in a porcine retina organ culture model.

Journal of Cellular and Molecular Medicine
José HurstStephanie C Joachim

Abstract

Nitrite oxide plays an important role in the pathogenesis of various retinal diseases, especially when hypoxic processes are involved. This degeneration can be simulated by incubating porcine retinal explants with CoCl2 . Here, the therapeutic potential of iNOS-inhibitor 1400W was evaluated. Degeneration through CoCl2 and treatment with the 1400W were applied simultaneously to porcine retinae explants. Three groups were compared: control, CoCl2 , and CoCl2  + iNOS-inhibitor (1400W). At days 4 and 8, retinal ganglion cells (RGCs), bipolar, and amacrine cells were analysed. Furthermore, the influence on the glia cells and different stress markers were evaluated. Treatment with CoCl2 resulted in a significant loss of RGCs already after 4 days, which was counteracted by the iNOS-inhibitor. Expression of HIF-1α and its downstream targets confirmed the effective treatment with 1400W. After 8 days, the CoCl2 group displayed a significant loss in amacrine cells and also a drastic reduction in bipolar cells was observed, which was prevented by 1400W. The decrease in microglia could not be prevented by the inhibitor. CoCl2 induces strong degeneration in porcine retinae by mimicking hypoxia, damaging certain retinal cell types. Treatment ...Continue Reading

References

Jan 14, 1994·Biochemical and Biophysical Research Communications·O GoureauY Courtois
Jan 5, 1995·Biochemical and Biophysical Research Communications·G A MurrellR J Williams
Sep 1, 1996·Vision Research·I M GoldsteinS Roth
Jun 1, 1997·Stroke; a Journal of Cerebral Circulation·A F SamdaniV L Dawson
Nov 20, 1997·The Journal of Clinical Investigation·C Nathan
Nov 20, 1997·The Journal of Clinical Investigation·K S Christopherson, D S Bredt
Mar 24, 1998·General Pharmacology·C B DuarteA P Carvalho
May 20, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S A Lyons, H Kettenmann
Oct 18, 2000·Survey of Ophthalmology·S BeattyM Boulton
Apr 28, 2001·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·G C Chiou
Mar 29, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Myriam BernaudinPascale Schumann-Bard
Jun 22, 2002·Experimental Eye Research·Anita Hendrickson, David Hicks
Sep 17, 2003·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·N FeltgenL Hansen
Oct 12, 2004·Current Drug Targets·Erik Schuetz, Solon Thanos
Dec 21, 2005·Reproduction, Fertility, and Development·Francesca GrasselliFederico Bianco
Jan 18, 2006·Mutation Research·Alberto IzzottiSergio C Saccà
Mar 1, 2006·Investigative Ophthalmology & Visual Science·Charanjit KaurWallace S Foulds
Jan 11, 2007·Documenta Ophthalmologica. Advances in Ophthalmology·Márta JanákyGyörgy Benedek
May 21, 2008·Free Radical Biology & Medicine·Mutay AslanIclal Yucel
Oct 22, 2008·Progress in Brain Research·S C Saccà, A Izzotti
Nov 27, 2009·Diabetes Research and Clinical Practice·Mae Sheikh-AliArshag D Mooradian
Jul 7, 2011·Human & Experimental Toxicology·S CatalaniP Apostoli
Dec 3, 2011·Brain Research·Alex H VielmaOliver Schmachtenberg
Nov 30, 2013·Free Radical Research·F CervellatiG Valacchi
Mar 29, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Shinji TsuchidaToshikazu Kubo
Apr 8, 2015·Breast Cancer Research : BCR·Sergio Granados-PrincipalJenny C Chang
Apr 14, 2015·Journal of Visualized Experiments : JoVE·Kai JanuschowskiSven Schnichels
Nov 5, 2015·ALTEX·Mardas DaneshianMarcel Leist
Feb 12, 2017·Experimental Cell Research·Hiroki MikamiYuji Nakayama
Apr 10, 2017·Acta Ophthalmologica·Radka OpatrilovaKatia Goncalves Dos Santos
Apr 15, 2017·Alternatives to Laboratory Animals : ATLA·José HurstStephanie C Joachim

❮ Previous
Next ❯

Citations

Dec 4, 2020·Neural Regeneration Research·Sven Schnichels, Stephanie C Joachim
Dec 17, 2020·Frontiers in Neuroscience·José HurstSven Schnichels

❮ Previous
Next ❯

Datasets Mentioned

BETA
KM035791.1

Methods Mentioned

BETA
PCR
protein folding

Software Mentioned

Primer3
GraphPad Prism
Statistica

Related Concepts

Related Feeds

Bipolar Disorder

Bipolar disorder is characterized by manic and/or depressive episodes and associated with uncommon shifts in mood, activity levels, and energy. Discover the latest research this illness here.