iNOS is not responsible for RyR1 S-nitrosylation in mdx mice with truncated dystrophin.

BMC Musculoskeletal Disorders
Ken'ichiro NogamiShin'ichi Takeda

Abstract

Previous research indicated that nitric oxide synthase (NOS) is the key molecule for S-nitrosylation of ryanodine receptor 1 (RyR1) in DMD model mice (mdx mice) and that both neuronal NOS (nNOS) and inducible NOS (iNOS) might contribute to the reaction because nNOS is mislocalized in the cytoplasm and iNOS expression is higher in mdx mice. We investigated the effect of iNOS on RyR1 S-nitrosylation in mdx mice and whether transgenic expression of truncated dystrophin reduced iNOS expression in mdx mice or not. Three- to 4-month-old C57BL/6 J, mdx, and transgenic mdx mice expressing exon 45-55-deleted human dystrophin (Tg/mdx mice) were used. We also generated two double mutant mice, mdx iNOS KO and Tg/mdx iNOS KO to reveal the iNOS contribution to RyR1 S-nitrosylation. nNOS and iNOS expression levels in skeletal muscle of these mice were assessed by immunohistochemistry (IHC), qRT-PCR, and Western blotting. Total NOS activity was measured by a citrulline assay. A biotin-switch method was used for detection of RyR1 S-nitrosylation. Statistical differences were assessed by one-way ANOVA with Tukey-Kramer post-hoc analysis. mdx and mdx iNOS KO mice showed the same level of RyR1 S-nitrosylation. Total NOS activity was not changed in...Continue Reading

References

Aug 1, 1992·The Journal of Experimental Medicine·H J ChoC Nathan
Jun 1, 1992·Cell Calcium·H H SchmidtF Murad
Apr 1, 1995·Journal of the Neurological Sciences·C Pastoret, A Sebille
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·W J ChangJ T Stull
Feb 15, 2001·Nature Cell Biology·S R JaffreyS H Snyder
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Douglas T HessJonathan S Stamler
Feb 12, 2008·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Akinori NakamuraShu-Ichi Ikeda
Mar 15, 2008·The Journal of Gene Medicine·Jun TanihataShin'ichi Takeda
Feb 10, 2009·Nature Medicine·Andrew M BellingerAndrew R Marks
May 17, 2012·Human Molecular Genetics·Christel GentilFrance Piétri-Rouxel
Aug 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yoshitsugu AokiShin'ichi Takeda
Sep 1, 2015·Journal of Biochemistry·Michihiro ImamuraShin'ichi Takeda
Sep 22, 2018·Biochemical and Biophysical Research Communications·Jun TanihataShin'ichi Takeda

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
dissection
electrophoresis
protein assay
pull down
co-immunoprecipitation

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.