Inosculation of blood vessels allows early perfusion and vitality of bladder grafts--implications for bioengineered bladder wall

Tissue Engineering. Part a
Stephanie L OsbornEric A Kurzrock

Abstract

Bioengineered bladder tissue is needed for patients with neurogenic bladder disease as well as for cancer. Current technologies in bladder tissue engineering have been hampered by an inability to efficiently initiate blood supply to the graft, ultimately leading to complications that include graft contraction, ischemia, and perforation. To date, the biological mechanisms of vascularization on transplant have not been suitably investigated for urologic tissues. To better understand the mechanisms of neovascularization on bladder wall transplant, a chimeric mouse model was generated such that angiogenesis and vasculogenesis could be independently assessed in vivo. Green fluorescence protein (GFP) transgenic mice received bone marrow transplants from β-galactosidase (LacZ) transgenic animals and then subsequent bladder wall transplants from wild-type donor mice. Before euthanization, the aorta was infused with fluorescent microbeads (fluorospheres) to identify perfused vessels. The contributions of GFP (angiogenesis) and LacZ (vasculogenesis) to the formation of CD31-expressing blood vessels within the wild-type graft were evaluated by immunohistochemistry at different time points and locations within the graft (proximal, middle, ...Continue Reading

References

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Citations

Dec 29, 2015·Current Bladder Dysfunction Reports·Bradley C GillChristopher J Chermansky
Sep 19, 2015·Current Opinion in Urology·Nikolai A SopkoTrinity J Bivalacqua
Jun 14, 2015·The Journal of Urology·Stephanie L Osborn, Eric A Kurzrock
Sep 16, 2017·Pediatric Research·Philip M IannacconeArun K Sharma
Jul 13, 2019·Vascular Specialist International·Sangil MinJames J Yoo
May 26, 2017·Biomedicines·Yvonne Y ChanStephanie L Osborn
Apr 29, 2021·Tissue Engineering. Part a·Toshiyuki KawaiYunzhi P Yang

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Methods Mentioned

BETA
Flow cytometry
transgenic
Fluorescence

Software Mentioned

ImageJ
R package lme4
Analyze Particles
R Core
R

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