Abstract
Inositol triphosphate (IP3) formation and increase in intracytoplasmic calcium are mediators of signal transduction in lymphocytes. It has been proposed that IP3 induces Ca2+ release from intracellular stores. It is in order to study the relationship between these two events that we have analyzed the effect of IP3 addition on Ca2+ mobilization in permeabilized resting T and B lymphocytes, EBV-B lymphocytes, and HTLV1-T lymphocytes. IP3 induces a rapid and significant release of Ca2+ from the endoplasmic reticulum in a dose-dependent manner. Ca2+ release is more sensitive to IP3 addition in cycling cells (EBV-B lymphocytes and HTLV1-T lymphocytes) than in resting T and B lymphocytes. Arachidonic acid (AA) induces Ca2+ release from the endoplasmic reticulum (ER) in a manner similar to that of IP3. Neither component has an effect on Ca2+ accumulated in mitochondria, and they have no additive effects suggesting that they act on a similar Ca2+ pool. These results directly demonstrate that in T and B human lymphocytes IP3 mobilizes Ca2+ from ER as in other cellular systems and that other potential second messengers, namely AA, could play a significant role in the internal mobilization of calcium during T and B lymphocyte activation.
References
Jan 1, 1985·European Journal of Immunology·K O'FlynnD C Linch
Dec 7, 1985·Lancet·E VilmerM Prieur
Jan 1, 1985·Annual Review of Biochemistry·L E Hokin
Mar 1, 1987·European Journal of Immunology·M T DimancheB Lisowska-Grospierre
May 1, 1985·The Biochemical Journal·B A WolfM L McDaniel
Mar 1, 1985·The Journal of Experimental Medicine·J B Imboden, J D Stobo
Aug 15, 1984·The Biochemical Journal·M J BerridgeK D Brown
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M VolpiR I Sha'afi
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M J WeissE L Reinherz
Oct 15, 1984·The Biochemical Journal·T J BidenC B Wollheim
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·Z DarzynkiewiczM R Melamed
Feb 1, 1984·European Journal of Immunology·J H KehrlA S Fauci
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A WeissJ Stobo
Oct 1, 1981·Clinical Immunology and Immunopathology·M K Dagg, D Levitt
Jun 7, 1984·Nature·M PrentkiC B Wollheim
Aug 15, 1984·The Biochemical Journal·R F IrvineM J Berridge
Nov 3, 1983·Nature·H StrebI Schulz
Aug 1, 1982·The Journal of Cell Biology·T PozzanT J Rink
Citations
Apr 28, 1995·Molecular and Cellular Endocrinology·F HertelendyJ Rigó
Mar 1, 1995·Cellular Signalling·W A KhanY A Hannun
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·M OikeB Nilius
May 12, 2000·Critical Care Medicine·E M Bulger, R V Maier
Jan 1, 1993·Prostaglandins, Leukotrienes, and Essential Fatty Acids·C SumidaE Nunez
Mar 1, 1994·Cell Calcium·L WuG R Brown
Sep 21, 2016·Scientific Reports·Kerstin HoppeWerner Klingler
Nov 1, 1995·Scandinavian Journal of Clinical and Laboratory Investigation·A T HøstmarkK Laake
Mar 22, 2003·The Journal of Pharmacology and Experimental Therapeutics·Peter H SchaferDavid I Stirling
Nov 1, 1995·Scandinavian Journal of Clinical and Laboratory Investigation·E W JensenN J Christensen
May 4, 1999·FEMS Immunology and Medical Microbiology·N SharmaN K Ganguly
Jun 26, 1998·Progress in Lipid Research·S K Jackson