Inositol 1,4,5-trisphosphate and basal Ca2+ release is affected by the cytoplasmic concentration of Cl- in endothelial cells

Biochemical and Biophysical Research Communications
P G Wood, J I Gillespie

Abstract

The effects of varying Cl- concentration in the intracellular bathing medium, on IP3-induced 45Ca2+ release from internal stores, were examined in saponin-permeabilised bovine aortic endothelial (BAE) cells. Results from this study show that the release of Ca2+ from the internal stores is affected by the cytoplasmic concentration of Cl- ions. Complete replacement of Cl- with gluconate augmented IP3 (3 microM)-induced 45Ca2+ release by 33 +/- 8%. Replacement of both Cl- and K+ with gluconate and NMG, respectively, had no significant effect on 45Ca2+ release. However, resting levels of internal 45Ca2+ were found to be affected by Cl- removal. These data suggest that in BAE cells, IP3 and also basal 45Ca2+ release may be regulated by the physiological intracellular Cl- concentration.

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Citations

Jun 4, 1998·Biochemical and Biophysical Research Communications·P G Wood, J I Gillespie

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