Inositol 1,4,5-trisphosphate-dependent oscillations of luminal [Ca2+] in permeabilized HSY cells.

The Journal of Biological Chemistry
A Tanimura, R James Turner

Abstract

Oscillations in intracellular Ca2+ concentration ([Ca2+]i) are thought to play an important role in phosphoinositide-linked Ca2+ signaling events. We demonstrate corresponding inositol 1,4, 5-trisphosphate (IP3)-dependent oscillations of Ca2+ concentration within the lumen of the IP3-sensitive stores ([Ca2+]L) of saponin-permeabilized HSY cells by monitoring [Ca2+]L with the fluorescent Ca2+ indicator Mag-fura-2. The associated openings and closings of the IP3-sensitive Ca2+ release channel were detected via quenching of Mag-fura-2 fluorescence due to the entry of Mn2+, a Ca2+ surrogate, into the stores. Evidence for complimentary Ca2+ oscillations at the external surface of the stores was provided by the membrane-bound Ca2+ probe Calcium Green C18. The permeabilization of saponin-treated HSY cells to macromolecules was confirmed by demonstrating that permeabilized cells readily took up and lost (t1/2 approximately 46 s) a fluorescently tagged 70-kDa dextran. Our results impose a number of constraints on possible mechanisms for [Ca2+]i oscillations. In addition, they support recent proposals that [Ca2+]i oscillations arise directly from the (biphasic) effects of Ca2+ itself on IP3-sensitive Ca2+ channel activity.

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Citations

Jul 27, 2000·Molecular Reproduction and Development·A MinelliI Mezzasoma
Sep 22, 2000·The Biochemical Journal·T J FitzsimmonsS J Pandol
Jan 1, 2009·The Journal of Medical Investigation : JMI·Akihiko TanimuraYosuke Tojyo
Jul 12, 2014·Mathematical Biosciences·James SneydDavid Yule
Jan 3, 2015·Biochimica Et Biophysica Acta·László Pecze, Beat Schwaller
Feb 22, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·I S Ambudkar
Feb 16, 2017·PLoS Computational Biology·Jung Min HanJames Sneyd
Jul 24, 2004·The Journal of Biological Chemistry·Akihiko TanimuraYosuke Tojyo
Jul 13, 2018·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Akihiko TanimuraKaori Murata
Feb 16, 2006·American Journal of Physiology. Endocrinology and Metabolism·John R PepperellMaricruz Merino
May 13, 1999·The Journal of Biological Chemistry·G HajnóczkyA P Thomas
May 16, 2000·The Journal of Biological Chemistry·G SzalaiG Hajnóczky
Aug 12, 1998·The American Journal of Physiology·J M HyvelinJ P Savineau

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