Inositol 1,4,5-trisphosphate induced Ca2+ release from chloroquine-sensitive and -insensitive intracellular stores in the intraerythrocytic stage of the malaria parasite P. chabaudi

Biochemical and Biophysical Research Communications
A P Passos, C R Garcia

Abstract

Isolated P. chabaudi parasites were permeabilized with digitonin and the function of intracellular Ca2+ stores was studied using the Ca2+ indicators arsenazo III or Fluo 3-acid in the medium. Addition of the second messenger InsP3 (5 microM) to permeabilized parasites leads to Ca2+ release into the medium, with the mean extent of release being 40 nmol Ca2+/10(8) cells. This Ca2+ release was completely abolished in the presence of heparin, an InsP3 receptor antagonist. The amount of Ca2+ released was approximately 50% reduced when InsP3 was added subsequent to the discharge of the endoplasmic reticulum (ER) Ca2+ pool with the SERCA (sarcoplasmic ER Ca2+ ATPase) inhibitors thapsigargin and tBHQ (2,5-di(ter-butyl)-1,4 benzohydroquinone). The thapsigargin- and tBHQ-sensitive pool account for 20 nmol of Ca2+/10(8) cells. If InsP3 was added after the discharge of the residual Ca2+ by addition of either the K+/H+ uncoupler nigericin or the antimalarial drug chloroquine, no further Ca2+ release was observed. This is the first report of InsP3-induced Ca2+ release in a parasite protozoa. In addition our finding that chloroquine depletes an InsP3-sensitive Ca2+ compartment, raises the possibility that the InsP3-dependent Ca2+ release from...Continue Reading

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Citations

Apr 22, 2009·Molecular and Cellular Biology·Eva-Maria LadenburgerHelmut Plattner
Sep 16, 2009·Therapeutics and Clinical Risk Management·Luiz Ma CarmargoCélia Rs Garcia
Apr 16, 2003·The Journal of Cell Biology·Marcos L GazariniCélia R S Garcia
Nov 6, 2012·Molecular and Biochemical Parasitology·Carina StritzkeGeorg W Mayr
Mar 14, 2007·Journal of Pineal Research·Alexandre BuduCélia Regina da Silva Garcia
Nov 11, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Piero BagnaresiCélia R S Garcia
Oct 22, 2015·Biological Reviews of the Cambridge Philosophical Society·Helmut Plattner
Jun 15, 2007·Memórias do Instituto Oswaldo Cruz·Marcos L GazariniCélia R S Garcia
Jan 25, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·R Sartorello, C R S Garcia
Sep 11, 2004·Biochemical and Biophysical Research Communications·Marcos L Gazarini, Célia R S Garcia
Oct 24, 2001·Journal of Biological Rhythms·C R GarciaL Madeira
Sep 17, 2015·The Journal of Biological Chemistry·Lucas Borges-PereiraSilvia N J Moreno
Jun 9, 2007·Parasitology Research·Thanaporn Rungruang, Sebastian Krystian Klosek
Dec 24, 2005·Physiological Reviews·Rosario Rizzuto, Tullio Pozzan
Oct 10, 2014·The Journal of Veterinary Medical Science·Ehab MossaadShin-Ichiro Kawazu
Mar 18, 2003·The Journal of Biological Chemistry·Ariel SavinaMaria I Colombo
Jun 10, 2009·Biotechnology Journal·Petra Rohrbach
Mar 29, 2001·Physiological Reviews·K Kirk
Oct 26, 2011·Essays in Biochemistry·Silvia N J MorenoDouglas A Pace
Jun 25, 2003·Biochemical and Biophysical Research Communications·Luciana MadeiraCélia R S Garcia
Aug 28, 2003·Current Opinion in Microbiology·Silvia N J Moreno, Roberto Docampo
Apr 7, 2005·Molecular and Biochemical Parasitology·Shirley L FariasCélia R S Garcia
May 23, 2006·Biochimica Et Biophysica Acta·Bikramjit RaychaudhurySalil C Datta
Apr 28, 2009·Molecular and Biochemical Parasitology·Fernanda C KoyamaCélia R S Garcia

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