PMID: 8610112Apr 2, 1996Paper

Inositol 1,4,5-trisphosphate receptor subtype 3 in pancreatic islet cell secretory granules revisited

Proceedings of the National Academy of Sciences of the United States of America
M RavazzolaL Orci

Abstract

It has been reported that the inositol 1,4,5-trisphosphate receptor subtype 3 is expressed in islet cells and is localized to both insulin and somatostatin granules [Blondel, O., Moody, M. M., Depaoli, A. M., Sharp, A. H., Ross, C. A., Swift, H. & Bell, G. I. (1994) Proc. Natl. Acad. Sci. USA 91, 7777-7781]. This subcellular localization was based on electron microscope immunocytochemistry using antibodies (affinity-purified polyclonal antiserum AB3) directed to a 15-residue peptide of rat inositol trisphosphate receptor subtype 3. We now show that these antibodies cross-react with rat, but not human, insulin. Accordingly, the anti-inositol trisphosphate receptor subtype 3 (AB3) antibodies label electron dense cores of mature (insulin-rich) granules of rat pancreatic beta cells, and rat granule labeling was blocked by preabsorption of the AB3 antibodies with rat insulin. The immunostaining of immature, Golgi-associated proinsulin-rich granules with AB3 antibodies was very weak, indicating that cross-reactivity is limited to the hormone and not its precursor. Also, the AB3 antibodies labeled pure rat insulin crystals grown in vitro but failed to stain crystals grown from pure human insulin. By immunoprecipitation, the antibodies...Continue Reading

References

Jan 1, 1992·Annual Review of Physiology·C D Ferris, S H Snyder
Sep 21, 1989·Nature·M J Berridge, R F Irvine
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L OrciA Perrelet
Apr 1, 1982·Journal of Microscopy·B L ArmbrusterW Villiger
Jul 1, 1980·The Histochemical Journal·K T Tokuyasu
Aug 1, 1995·Trends in Neurosciences·J Meldolesi, T Pozzan
Aug 1, 1995·Trends in Neurosciences·C Colaco
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·O BlondelG I Bell
Aug 1, 1994·Cell Calcium·G A RutterC B Wollheim
Jan 28, 1993·Nature·M J Berridge

❮ Previous
Next ❯

Citations

Nov 4, 2010·Cellular and Molecular Neurobiology·Seung Hyun YooYong Suk Hur
Jan 1, 2009·The Journal of Biological Chemistry·Christian RoskerColin W Taylor
Oct 20, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Seung Hyun Yoo
Nov 20, 2002·Annals of the New York Academy of Sciences·Seung Hyun YooHee Yun Park
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M SrivastavaH B Pollard
Sep 23, 2014·Cell Calcium·Patrick GilonMagalie A Ravier
Jul 9, 2010·Trends in Endocrinology and Metabolism : TEM·Jakob Suckale, Michele Solimena
Jul 1, 1997·Cell Calcium·S Muallem, M G Lee
Jan 17, 2015·Cell Calcium·Sandip Patel, Xinjiang Cai
Feb 11, 2000·Cell Calcium·C W TaylorS A Morris
Jul 8, 2003·Microscopy Research and Technique·Gertrud GopingRichard Leapman
Nov 4, 2006·The Journal of Physiological Sciences : JPS·Yutaka EndoMasumi Inoue
Sep 16, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Santanu Banerjee, Gaiti Hasan
Dec 24, 2005·Physiological Reviews·Rosario Rizzuto, Tullio Pozzan
Feb 23, 2013·Pharmacological Reviews·Christopher J Barker, Per-Olof Berggren
Jul 21, 1998·The Journal of Biological Chemistry·W J ScheenenC Fasolato
Aug 27, 2021·Metabolites·Mark GermanosMelkam A Kebede

❮ Previous
Next ❯