Inositol depletion restores vesicle transport in yeast phospholipid flippase mutants

PloS One
Kanako YamagamiKazuma Tanaka

Abstract

In eukaryotic cells, type 4 P-type ATPases function as phospholipid flippases, which translocate phospholipids from the exoplasmic leaflet to the cytoplasmic leaflet of the lipid bilayer. Flippases function in the formation of transport vesicles, but the mechanism remains unknown. Here, we isolate an arrestin-related trafficking adaptor, ART5, as a multicopy suppressor of the growth and endocytic recycling defects of flippase mutants in budding yeast. Consistent with a previous report that Art5p downregulates the inositol transporter Itr1p by endocytosis, we found that flippase mutations were also suppressed by the disruption of ITR1, as well as by depletion of inositol from the culture medium. Interestingly, inositol depletion suppressed the defects in all five flippase mutants. Inositol depletion also partially restored the formation of secretory vesicles in a flippase mutant. Inositol depletion caused changes in lipid composition, including a decrease in phosphatidylinositol and an increase in phosphatidylserine. A reduction in phosphatidylinositol levels caused by partially depleting the phosphatidylinositol synthase Pis1p also suppressed a flippase mutation. These results suggest that inositol depletion changes the lipid c...Continue Reading

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Citations

Oct 1, 2016·Cell Adhesion & Migration·Katharina B Beer, Ann Marie Wehman
Feb 6, 2017·European Journal of Cell Biology·Anna D FrejRobin S B Williams
Apr 14, 2017·Molecular Biology of the Cell·Lauren E DaltonElizabeth Conibear
Oct 4, 2016·Applied and Environmental Microbiology·Arpita SenJeremy Thorner
Jan 7, 2017·G3 : Genes - Genomes - Genetics·Takaharu YamamotoKazuma Tanaka

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Methods Mentioned

BETA
PCR
dissection
density gradient fractionation
glycosylation

Software Mentioned

AQUACOS
MOS
Analyst

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