Inositol triphosphate participates in an oestradiol nongenomic signalling pathway involved in accelerated oviductal transport in cycling rats

The Journal of Endocrinology
Pedro A OrihuelaH B Croxatto

Abstract

Oestradiol (E(2)) accelerates oviductal transport of oocytes in cycling rats through a nongenomic pathway that involves the cAMP-PKA signalling cascade. Here we examined the role of the inositol triphosphate (IP3) and mitogen-activated protein kinase (MAPK) signalling cascades in this nongenomic pathway. Oestrous rats were injected with E(2) s.c. and intrabursally (i.b) with the selective inhibitors of phospholipase C (PLC) ET-18-OCH(3) or MAPK PD98059. The number of eggs in the oviduct assessed 24 h later showed that ET-18-OCH(3) blocked E(2)-induced egg transport acceleration, whereas PD98059 had no effect. Other oestrous rats were treated with E(2) s.c. and 1, 3 or 6 h later oviducts were excised and the levels of IP3 and phosphorylated MAPK p44/42 (activated) were determined by radioreceptor assay and western blot, respectively. Oestradiol administration increased IP3 level at 1 and 6 h after treatment, whereas activated MAPK p44/42 level was unchanged. Finally, we explored whether cAMP-PKA and PLC-IP3 signalling cascades are coupled. Inhibition of adenylyl cyclase by i.b. injection of SQ 22536 blocked the increase of IP3 levels induced by E(2), while inhibition of PLC by ET-18-OCH(3) had no effect on E(2)-induced PKA activ...Continue Reading

Citations

Dec 2, 2009·Reproductive Biology and Endocrinology : RB&E·Pedro A OrihuelaHoracio B Croxatto
Dec 25, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Alexis Parada-BustamanteHoracio B Croxatto
Nov 14, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·María L OrósticaPedro A Orihuela
Jul 21, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·María L OrósticaPedro A Orihuela
Jul 22, 2014·Journal of Clinical Pharmacology·Simona DinicolaMariano Bizzarri
Nov 2, 2014·Molecular Reproduction and Development·María L OrósticaPedro A Orihuela
Jul 15, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Patricia ReuquénPedro A Orihuela
Oct 26, 2016·Molecular Reproduction and Development·Alexis Parada-BustamantePedro A Orihuela
Jun 6, 2014·Expert Review of Clinical Pharmacology·Vittorio Unfer, Giuseppina Porcaro
Nov 23, 2016·The Journal of Endocrinology·Shuai Li, Wipawee Winuthayanon
Oct 2, 2020·International Journal of Molecular Sciences·Vittorio UnferMariano Bizzarri
May 13, 2020·Trends in Endocrinology and Metabolism : TEM·Fabio FacchinettiUNKNOWN Group of 'Inositol in PCOS and Reproduction'

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