Inotilone from Inonotus linteus suppresses lung cancer metastasis in vitro and in vivo through ROS-mediated PI3K/AKT/MAPK signaling pathways

Scientific Reports
Wei ChaoGuan-Jhong Huang

Abstract

Metastasis is one of the main causes of mortality in cancer patients. Inotilone, a major component of Inonotus linteus, is a traditional Chinese medical herb. In this study, MTT results showed that inotilone had no obvious cytotoxicity. Animal model results revealed that inotilone suppressed cancer metastatic efficacy. Serum results showed that inotilone reduced the activity of matrix metalloproteinase (MMP)-2 and -9 and tumor necrosis factor alpha (TNF-α) activity as well as NO content. Additionally, inotilone affected MMP-9 and tissue inhibitor of metalloproteinase (TIMP)-2 protein expression and improved the activity of the antioxidant enzymes in the lung tissues of LLC-bearing mice. In addition, cell experimental results showed that inotilone reduced the activity of MMP-2/-9 and inhibited the ability for cellular migration and invasion. Inotilone decreased interleukin (IL)-8 expression in A549 cells. Western blot results revealed that inotilone affected the protein expression of MMPs, nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, anti-oxidant enzymes, mitogen activated protein kinase (MAPK), focal adhesion kinase (FAK), phosphoinositide-3 kinase (PI3K)-AKT, and nuclear factor (NF)κB. Therefore, we propose that inoti...Continue Reading

References

Feb 9, 1973·Science·J T RotruckW G Hoekstra
Jan 1, 1984·Methods in Enzymology·H Aebi
Apr 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D Weinstat-Saslow, P S Steeg
Mar 31, 1993·Biochemical and Biophysical Research Communications·M P ShermanL J Ignarro
May 1, 1994·Analytical Biochemistry·D E Kleiner, W G Stetler-Stevenson
Feb 13, 2001·The Journal of Clinical Investigation·A S Baldwin
Dec 20, 2002·Nature·Lisa M Coussens, Zena Werb
Apr 20, 2005·Cancer Letters·Julien MazieresDavid M Jablons
May 24, 2005·Biochimica Et Biophysica Acta·Mikael Björklund, Erkki Koivunen
Feb 14, 2006·Clinical & Experimental Metastasis·Anne LewisJoseph J Cullen
Nov 30, 2006·Molecular Carcinogenesis·Jinjin GuoChang-Yan Chen
Dec 8, 2006·The Biochemical Journal·Barry Halliwell
Dec 19, 2006·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·F LuppiP S Hiemstra
Sep 16, 2010·Free Radical Biology & Medicine·Simone ReuterBharat B Aggarwal
Apr 2, 2011·Journal of Agricultural and Food Chemistry·Guan-Jhong HuangYueh-Hsiung Kuo
Jun 26, 2012·International Journal of Cancer. Journal International Du Cancer·Lin Yan
Dec 12, 2012·Journal of Clinical Immunology·Juliana Candido, Thorsten Hagemann
Jun 3, 2014·BMC Complementary and Alternative Medicine·Weidong LiBaojin Hua
Nov 2, 2017·Nature Reviews. Urology·Karen S SfanosAngelo M De Marzo

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Citations

Oct 28, 2019·British Journal of Pharmacology·Huihong ZengLijian Shao
Jan 9, 2020·Journal of Cancer Research and Clinical Oncology·Cheng ZhangLin Shen
Aug 28, 2020·Organic Letters·Ángel Rentería-GómezJieping Zhu

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Methods Mentioned

BETA
Nuclear Magnetic Resonance
zymography
ELISA
xenografts
nuclear translocation

Software Mentioned

SPSS
Kodak Molecular Imaging

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