Insect exclusion limits variation in bacterial microbiomes of tomato flowers and fruit

Journal of Applied Microbiology
Sarah M AllardShirley A Micallef

Abstract

The effect of insect exclusion via netting on bacterial microbiota associated with field-grown tomato fruit and flowers was evaluated. Amplicon-based bacterial community profiling from insect-exposed plants and plants wrapped in nylon mosquito netting was conducted on total DNA extracted from tomato flower and mature unripe fruit washes. The V1-V3 region of the 16S rRNA gene was sequenced using Illumina MiSeq and analyzed using QIIME v1.8. The carposphere supported significantly more phylogenetic diversity compared to the anthosphere, as measured by Operational Taxonomic Unit (OTU) richness (p=0.001) and Faith's Phylogenetic Diversity (p=0.004). Flowers and fruit hosted distinct bacterial community structures (R2 =0.27, p=0.001), with specific taxonomic differences in taxa that included the Xanthomonadaceae (higher in flowers), and the Pseudomonadaceae, Methylobacteriaceae and Rhizobiales (higher in fruit) (FDR-p<0.05). Bacterial community profiles of netted plants were overall statistically similar to non-netted plants for both flowers and fruit (p>0.10). However, less variation between samples was observed among flowers (~50% less, p=0.004) and green fruit (~10% less, p=0.038) collected from netted than non-netted plants. Ins...Continue Reading

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Citations

Mar 25, 2020·Microbial Biotechnology·Youming ShenHaifei Li
Aug 24, 2019·The New Phytologist·María Rebolleda-GómezTia-Lynn Ashman
Feb 6, 2020·Scientific Reports·Sarah M AllardShirley A Micallef
Oct 9, 2021·Proceedings of the National Academy of Sciences of the United States of America·Julien MassoniJulia A Vorholt
Nov 9, 2021·Frontiers in Microbiology·David Johnston-MonjeLuis Augusto Becerra Lopez-Lavalle

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