Insect olfactory receptors are heteromeric ligand-gated ion channels

Nature
Koji SatoKazushige Touhara

Abstract

In insects, each olfactory sensory neuron expresses between one and three ligand-binding members of the olfactory receptor (OR) gene family, along with the highly conserved and broadly expressed Or83b co-receptor. The functional insect OR consists of a heteromeric complex of unknown stoichiometry but comprising at least one variable odorant-binding subunit and one constant Or83b family subunit. Insect ORs lack homology to G-protein-coupled chemosensory receptors in vertebrates and possess a distinct seven-transmembrane topology with the amino terminus located intracellularly. Here we provide evidence that heteromeric insect ORs comprise a new class of ligand-activated non-selective cation channels. Heterologous cells expressing silkmoth, fruitfly or mosquito heteromeric OR complexes showed extracellular Ca2+ influx and cation-non-selective ion conductance on stimulation with odorant. Odour-evoked OR currents are independent of known G-protein-coupled second messenger pathways. The fast response kinetics and OR-subunit-dependent K+ ion selectivity of the insect OR complex support the hypothesis that the complex between OR and Or83b itself confers channel activity. Direct evidence for odorant-gated channels was obtained by outsid...Continue Reading

References

Apr 1, 1996·Chemical Senses·K E Kaissling
Mar 29, 2000·The Journal of General Physiology·M Murakami, H Kijima
Sep 6, 2000·The Journal of Biological Chemistry·C R HalaszovichA Luckhoff
Jun 8, 2001·Neuron·M de BruyneJ R Carlson
Jun 29, 2002·Science·Georg NagelPeter Hegemann
May 28, 2003·Biochemical and Biophysical Research Communications·Sayako KatadaKazushige Touhara
Jun 27, 2003·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·J KriegerH Breer
Jan 16, 2004·Nature·Elissa A HallemJohn R Carlson
Mar 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·R Jason PittsLaurence J Zwiebel
Jun 24, 2004·Cell·Elissa A HallemJohn R Carlson
Dec 14, 2004·Nature Neuroscience·Eva M NeuhausHanns Hatt
Feb 23, 2005·Current Biology : CB·Walton D JonesLeslie B Vosshall
Mar 8, 2005·Neuron·Aaron L GoldmanJohn R Carlson
Sep 6, 2005·Current Biology : CB·Africa CoutoBarry J Dickson
Sep 6, 2005·Current Biology : CB·Elane Fishilevich, Leslie B Vosshall
Feb 3, 2006·Protein Science : a Publication of the Protein Society·Markus WistrandErik L L Sonnhammer
Mar 24, 2006·Nature·William N Zagotta
Apr 18, 2006·Cell·Elissa A Hallem, John R Carlson
Nov 17, 2007·FEBS Letters·Carolina LundinIngMarie Nilsson

❮ Previous
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Citations

Sep 29, 2009·Die Naturwissenschaften·Simon Conway Morris
Mar 21, 2009·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Christian Flecke, Monika Stengl
Aug 22, 2009·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Karl-Ernst Kaissling
Dec 25, 2009·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Marien de BruyneCoral G Warr
Apr 9, 2013·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Karl-Ernst Kaissling
Jul 5, 2013·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Monika Stengl, Nico W Funk
Mar 5, 2013·Molecules and Cells·Walton Jones
Mar 9, 2013·Molecules and Cells·Anders Enjin, Greg Seong-Bae Suh
Jun 7, 2008·Journal of Chemical Ecology·M de Bruyne, T C Baker
Dec 12, 2012·Journal of Chemical Ecology·Wei XuAlisha Anderson
Mar 26, 2014·Cell Calcium·Latha MukundaDieter Wicher
Jun 28, 2005·Insect Biochemistry and Molecular Biology·Jonathan Bohbot, Richard G Vogt
Mar 25, 2011·Genetics Research·Robert R H Anholt
May 31, 2011·Journal of Proteome Research·Mao FengJianke Li
May 14, 2009·Heredity·A Sánchez-GraciaJ Rozas
Apr 28, 2009·Nature·Julia L Semmelhack, Jing W Wang
Sep 23, 2011·Nature·Maurizio PellegrinoLeslie B Vosshall
Nov 17, 2011·Nature Communications·Katsuhisa OzakiHiroshi Yoshikawa
Jan 11, 2011·Nature Neuroscience·Katherine I Nagel, Rachel I Wilson
Feb 18, 2010·Proceedings of the National Academy of Sciences of the United States of America·Guirong WangLaurence J Zwiebel
May 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Atulya IyengarObaid Siddiqi
Aug 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Nobuo MisawaShoji Takeuchi
Apr 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ewald Grosse-WildeBill S Hansson
Jun 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Koji SatoKazushige Touhara
May 11, 2011·Proceedings of the National Academy of Sciences of the United States of America·Patrick L JonesLaurence J Zwiebel
Jul 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Allison F Carey, John R Carlson
Aug 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Greg P LearyKevin W Wanner
Nov 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Maria OlmedoMartha Merrow
Feb 12, 2010·The Journal of Biological Chemistry·Andrew S Nichols, Charles W Luetje
Feb 4, 2009·Chemical Senses·Harland M PatchHugh M Robertson
Apr 24, 2009·Chemical Senses·Nicolas MeunierPhilippe Lucas
Nov 18, 2009·Chemical Senses·Robert R H Anholt, Taufika Islam Williams
Jun 4, 2010·Chemical Senses·Barry W Ache
Oct 14, 2010·Chemical Senses·Nico BredendiekEva M Neuhaus

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