PMID: 10741964Mar 31, 2000

Insect population control using a dominant, repressible, lethal genetic system

D D ThomasL S Alphey


A major modification to the sterile insect technique is described, in which transgenic insects homozygous for a dominant, repressible, female-specific lethal gene system are used. We demonstrate two methods that give the required genetic characteristics in an otherwise wild-type genetic background. The first system uses a sex-specific promoter or enhancer to drive the expression of a repressible transcription factor, which in turn controls the expression of a toxic gene product. The second system uses non-sex-specific expression of the repressible transcription factor to regulate a selectively lethal gene product. Both methods work efficiently in Drosophila melanogaster, and we expect these principles to be widely applicable to more economically important organisms.


Apr 14, 2010·Transgenic Research·Holly J FergusonMalcolm J Fraser
Oct 18, 2011·Molecular Biotechnology·Jiun-Shuan ChaoChiun-Jye Yuan
Sep 30, 2010·Journal of Genetics·Tarig H Dafa'allaL S Alphey
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Maria MarkakiCharalambos Savakis
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Kathryn A RaphaelMarianne Frommer
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Katia KomitopoulouAnastassios C Mintzas
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Alan S RobinsonPeter W Atkinson
Nov 19, 2013·Insect Biochemistry and Molecular Biology·Xiu-Ye WangYong-Zhen Xu
Mar 24, 2004·Trends in Parasitology·Yeya T TouréCarlos M Morel
Feb 24, 2001·Theriogenology·D W SilversidesJ Lussier
May 30, 2002·Molecular and Biochemical Parasitology·L S Alphey, Morten Andreasen
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Carsten HornErnst A Wimmer
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Peter W Atkinson
Sep 13, 2002·Insect Biochemistry and Molecular Biology·L S Alphey
Jun 8, 2002·Mutation Research·Alan S Robinson
Mar 2, 2002·Trends in Parasitology·K S AultmanE D Walker
Aug 7, 2003·Trends in Parasitology·Mark Benedict, Alan S Robinson
Nov 9, 2010·Nature Biotechnology·Bruce E TabashnikYves Carrière
Nov 1, 2011·Nature Biotechnology·Angela F HarrisL S Alphey
Feb 27, 2007·Nature Biotechnology·Guoliang FuL S Alphey
Dec 17, 2002·Nature Biotechnology·Carsten Horn, Ernst A Wimmer
Apr 5, 2007·Nature Protocols·Neil F LoboFrank H Collins
Nov 28, 2001·European Journal of Biochemistry·S O ZakharkinH Benes
Feb 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Guoliang FuL S Alphey
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Megan R Wise de ValdezWilliam C Black
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anjiang TanYongping Huang
Sep 4, 2009·Vector Borne and Zoonotic Diseases·L S AlpheyStephen L Dobson
Apr 18, 2009·Vector Borne and Zoonotic Diseases·L S Alphey
Oct 5, 2012·Clinical Microbiology Reviews·Tonya M ColpittsErol Fikrig
Dec 4, 2003·Annual Review of Entomology·Fred Gould, Paul Schliekelman
Sep 10, 2004·Annual Review of Entomology·Helen Pates, Christopher Curtis

Related Concepts

Genes, Insect
Msl-2 protein, Drosophila
Drosophila melanogaster Proteins
Genetic Conditions, Dominant
Egg White Proteins
v-Ki-ras Genes
Pest Control, Biological
YP3 protein, Drosophila
Fat Body

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