Insecticide cytotoxicity and CYP1A1/2 induction in primary human and rat hepatocyte cultures

Toxicology in Vitro : an International Journal Published in Association with BIBRA
G de SousaRoger Rahmani

Abstract

With the increasing demand for insecticide products, the question of their safety has become one of the serious world public health concerns. The capability of compounds belonging to the major insecticide families [such as chlorinated hydrocarbons (DDT), carbamates (carbaryl: CBR), organophosphorus compounds (malathion, tetrachlorvinfos: MAL, TCV), pyrethroids (cypermethrin: CPR) and benzoylurea (diflubenzuron: DFU)] in inducing CYP1 Al in rat and human hepatocyte cultures has been tested. Cells were treated during 3 days with six non-toxic increasing doses of insecticides and CYP1A1 expression was assessed by ethoxyresorufin O-deethylase (EROD) activity and by Northern blots. A strong and dose-dependent induction was observed with TCV and DFU, both in human (approx. five- and sevenfold over control, respectively) and in rat hepatocytes (approx. sevenfold). However, EROD induction and CYP/A1 mRNA levels were correlated for DFU but not for TCV, suggesting different regulation mechanisms for PCYP1A1 gene expression by the two compounds. CBR and CPR exerted less induction in both cell types (approx. 2.5-fold induction compared with approximately 16-fold for 3-methylcholanthrene), whereas DDT and MAL showed no action on human hepat...Continue Reading

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Citations

Oct 19, 2002·Chemico-biological Interactions·Jun TangErnest Hodgson
Dec 22, 1999·Chemico-biological Interactions·J N TetteyA R Pitt
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P J Dierickx
Feb 2, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Georges de SousaRoger Rahmani
Feb 9, 2012·Toxicology·Khaled AbassJukka Hakkola
Jul 3, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sushila PatelAlok Dhawan
Nov 7, 2006·Chemico-biological Interactions·Parikshit C DasRandy L Rose
Mar 15, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M-E WilleminC Brochot
Mar 30, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E MaranM J Ruiz
Dec 18, 2013·Chemico-biological Interactions·Rozenn JosseAndre Guillouzo
Jun 19, 2016·Toxicology and Applied Pharmacology·S TengA R R Pery
May 4, 2017·Journal of Biomolecular Structure & Dynamics·Mohd ShahbaazKrishna Bisetty
May 24, 2001·Archives of Biochemistry and Biophysics·N LiuL S Kaminsky

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