Insensitivity of chromosome I and the cell cycle to blockage of replication and segregation of Vibrio cholerae chromosome II.

MBio
Ryosuke Kadoya, Dhruba K Chattoraj

Abstract

Vibrio cholerae has two chromosomes (chrI and chrII) whose replication and segregation are under different genetic controls. The region covering the replication origin of chrI resembles that of the Escherichia coli chromosome, and both origins are under control of the highly conserved initiator, DnaA. The origin region of chrII resembles that of plasmids that have iterated initiator-binding sites (iterons) and is under control of the chrII-specific initiator, RctB. Both chrI and chrII encode chromosome-specific orthologs of plasmid partitioning proteins, ParA and ParB. Here, we have interfered with chrII replication, segregation, or both, using extra copies of sites that titrate RctB or ParB. Under these conditions, replication and segregation of chrI remain unaffected for at least 1 cell cycle. In this respect, chrI behaves similarly to the E. coli chromosome when plasmid maintenance is disturbed in the same cell. Apparently, no checkpoint exists to block cell division before the crippled chromosome is lost by a failure to replicate or to segregate. Whether blocking chrI replication can affect chrII replication remains to be tested. Chromosome replication, chromosome segregation, and cell division are the three main events of ...Continue Reading

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Citations

Mar 4, 2014·PLoS Genetics·Jong Hwan Baek, Dhruba K Chattoraj
Aug 12, 2014·Plasmid·Samuel Million-Weaver, Manel Camps
Nov 13, 2014·Nucleic Acids Research·Godefroid CharbonAnders Løbner-Olesen
Aug 11, 2017·Microbiology and Molecular Biology Reviews : MMBR·George C diCenzo, Turlough M Finan
May 26, 2018·PLoS Genetics·Revathy RamachandranDhruba K Chattoraj

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Methods Mentioned

BETA
PCR

Software Mentioned

Iterons
RctB

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