Insertion of a coiled-coil peptide from influenza virus hemagglutinin into membranes

Science
Y G YuY K Shin

Abstract

The trimeric protein hemagglutinin (HA) of the influenza viral envelope is essential for cell entry. To investigate the interaction of HA with membranes, two 40-residue, cysteine-substituted peptides comprising the loop region and the first part of the coiled-coil stem were synthesized and modified with a nitroxide spin label. Electron paramagnetic resonance analysis revealed that the peptide inserts reversibly into phospholipid vesicles under endosomal pH conditions. This result suggests that some or all of the long coiled-coil trimer of HA may insert into membranes, which could bring the viral and cell membranes closer together and facilitate fusion.

Citations

Nov 9, 2000·Cell Biology International·J Bentz, A Mittal
Sep 21, 2000·Folia Microbiologica·L Jindrák, L Grubhoffer
Aug 7, 2012·The Journal of Membrane Biology·Thomas PortetMarie-Pierre Rols
May 7, 2010·Protoplasma·Jennifer A ThorleyJoshua Zachary Rappoport
Aug 1, 1995·Current Opinion in Structural Biology·L V Chernomordik, J Zimmerberg
Oct 1, 1995·Current Opinion in Structural Biology·K A Karlsson
Jun 6, 2000·Advanced Drug Delivery Reviews·P J KlasseM Marsh
Mar 1, 1995·Current Biology : CB·F M Hughson
Jul 13, 2000·European Journal of Cancer Care·A E Murphy
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·J ChenD C Wiley
Mar 22, 2002·Journal of Virology·Fabiana A CarneiroAndrea T Da Poian
Jan 1, 1996·Annual Review of Cell and Developmental Biology·L D HernandezJ M White
Jun 19, 2001·Biological Chemistry·W van 't HofA V Amerongen
Feb 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Fumimasa NomuraKingo Takiguchi
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·C M CarrP S Kim
Dec 23, 2008·International Journal of Biological Macromolecules·Priyankar SenRizwan Hasan Khan
Jul 16, 2008·Chemistry : a European Journal·Ayumi KashiwadaToshiki Tanaka
Mar 25, 2011·Macromolecular Rapid Communications·Bojana ApostolovicHarm-Anton Klok
Mar 3, 2015·Advanced Materials·Georgina K SuchFrank Caruso
Feb 4, 1998·Biophysical Journal·T ShangguanJ Bentz
Jul 23, 2003·Biochimica Et Biophysica Acta·Joe Bentz, Aditya Mittal
Aug 31, 2000·Annual Review of Biochemistry·J J Skehel, D C Wiley
Feb 15, 1997·Journal of Colloid and Interface Science·S A Soltesz, D A Hammer
Aug 30, 2008·Biochimica Et Biophysica Acta·Jaime GuillénJosé Villalaín
Feb 1, 1996·Chemistry & Biology·D CharychR C Stevens
Mar 27, 2009·Chemical Communications : Chem Comm·Ayumi KashiwadaKiyomi Matsuda
Sep 1, 2000·Journal of Molecular Biology·Y KligerY Shai
Dec 30, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Ayumi KashiwadaKiyomi Matsuda
Aug 20, 2016·The Journal of Physical Chemistry. B·Xingcheng LinJosé N Onuchic
Feb 12, 1999·Journal of Molecular Biology·R F EpandR M Epand

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.