Insertion of an EYFP-pp71 (UL82) coding sequence into the human cytomegalovirus genome results in a recombinant virus with enhanced viral growth.

Journal of Virology
Nina TavalaiT Stamminger

Abstract

The human cytomegalovirus (HCMV) UL82-encoded tegument protein pp71 has recently been shown to activate viral immediate-early (IE) gene expression by neutralizing a cellular intrinsic immune defense instituted by the ND10 protein hDaxx. Pp71 localizes to ND10 upon infection and induces the degradation of hDaxx. Here, we report the successful generation of a recombinant HCMV expressing enhanced yellow fluorescent protein (EYFP) fused to the N terminus of pp71. Intriguingly, insertion of the EYFP-UL82 coding sequence into the HCMV AD169 genome gave rise to a recombinant virus, termed AD169/EYFP-pp71, that replicates to significantly higher titers than wild-type AD169. In particular, we noticed strongly increased protein levels of pp71 after AD169/EYFP-pp71 inoculation. Although the high abundance of pp71 resulted in augmented packaging of the tegument protein into viral particles, no increased hDaxx degradation was detectable upon AD169/EYFP-pp71 infection. In contrast, further investigation revealed a significantly enhanced viral DNA replication compared to wild-type AD169. Thus, we hypothesize that an as-yet-unidentified function of pp71 contributes to the enhanced infectivity of AD169/EYFP-pp71. This assumption is additionally...Continue Reading

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Citations

May 29, 2009·Virology Journal·Iain P NicholsonChris M Preston
Dec 5, 2014·Journal of Virology·Michael J Imperiale, Mengxi Jiang
Feb 26, 2016·Journal of Virology·Tim SchommartzWolfram Brune
Sep 2, 2011·The Journal of Infectious Diseases·Shannon A RossSuresh B Boppana
Dec 12, 2012·PloS One·Mirko CorteseMarcello Merola
Jun 2, 2016·Journal of Medical Virology·Kalpana DulalHua Zhu
Apr 19, 2016·Viruses·Heather A VincentNathaniel J Moorman
Jul 8, 2020·Proceedings of the National Academy of Sciences of the United States of America·Sonali ChaturvediLeor S Weinberger
Aug 29, 2018·Proceedings of the National Academy of Sciences of the United States of America·Noam VardiLeor S Weinberger

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