Insertion of bitopic membrane proteins into the inner membrane of mitochondria involves an export step from the matrix.

The Journal of Biological Chemistry
Frank BaumannJohannes M Herrmann

Abstract

The mitochondrial inner membrane contains a large number of polytopic proteins that are derived from prokaryotic ancestors of mitochondria. Little is known about the intramitochondrial sorting of these proteins. We chose two proteins of known topology as examples to study the pathway of insertion into the inner membrane; Mrs2 and Yta10 are bitopic proteins that expose negatively charged loops of different complexity into the intermembrane space. Here we show that both Mrs2 and Yta10 transiently accumulate as sorting intermediates in the matrix before they integrate into the inner membrane. The sorting pathway of both proteins can be separated into two sequential reactions: (i) import into the matrix and (ii) insertion from the matrix into the inner membrane. The latter process was found to depend on the membrane potential and, in this respect, is similar to the insertion of membrane proteins in bacteria. A comparison of the charge distribution of intermembrane space loops in a variety of mitochondrial inner membrane proteins suggests that this mode of "conservative sorting" might be the typical insertion route for polytopic inner membrane proteins that originated from bacterial ancestors.

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Citations

Feb 25, 2003·Trends in Microbiology·Johannes M Herrmann
Nov 4, 2005·Molecular Biology of the Cell·Cory D DunnRobert E Jensen
Jun 3, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Muhammad Bashir KhanKristina Djinović-Carugo
Feb 1, 2007·Annual Review of Biochemistry·Walter Neupert, Johannes M Herrmann
Oct 25, 2006·The Journal of Cell Biology·Ming Li, Danny J Schnell
May 18, 2007·FEBS Letters·Lea VojtaBettina Bölter
Feb 14, 2015·Journal of Molecular Biology·Walter Neupert
Jan 15, 2015·Toxicological Research·Aekyong Kim
Sep 11, 2007·Biophysical Journal·Rainer SchindlRudolf J Schweyen
Dec 4, 2004·Biochimica Et Biophysica Acta·Immo E SchefflerPrasanth Potluri
Jun 10, 2008·The Biochemical Journal·Takehito Inaba, Danny J Schnell

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