PMID: 8588974Aug 1, 1995Paper

Insertion of functional intact insulin receptors into hepatocytes

Cellular Signalling
K Christiansen, J Carlsen

Abstract

Isolated insulin receptors were inserted into hepatocytes by fusion with receptor-lysophospholipid micelles. The number of receptors was increased approximately two times as monitored by insulin binding experiments. Addition of exogenous receptors resulted in increased receptor autophosphorylation. The exogenous added receptors could be coupled to the intracellular effector system as shown by increased phosphorylation of the insulin receptor substrate 1, IRS-1, and by increased glycogen synthesis. The co-incorporated lysophospholipid was partly metabolized to phospholipid. This method for introducing insulin receptors into cells represents a novel system to study the pathway of insulin signalling and may provide information as to the role of variation of receptor number in insulin action in cellular metabolism of specific cells.

References

Mar 25, 1975·Biochimica Et Biophysica Acta·A Helenius, K Simons
Jan 15, 1992·The Biochemical Journal·K Christiansen, J Carlsen
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K ChristiansenJ Vinten
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P A WildenM F White
Jan 1, 1990·The International Journal of Biochemistry·T O'Hare, P F Pilch
Sep 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S R WangR Infante
Nov 9, 1983·Biochimica Et Biophysica Acta·K Christiansen, J Carlsen
Sep 14, 1982·Biochimica Et Biophysica Acta·P L Fox, D B Zilversmit
Jun 1, 1994·The Journal of Membrane Biology·J Tranum-JensenJ Vinten
Jul 1, 1994·Trends in Biochemical Sciences·M G MyersM F White
Aug 1, 1969·FEBS Letters·J I. Howell, J A. Lucy

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