Insertion of inverted Ter sites into the terminus region of the Escherichia coli chromosome delays completion of DNA replication and disrupts the cell cycle

Molecular Microbiology
B Sharma, Thomas M Hill

Abstract

To investigate the co-ordination between DNA replication and cell division, we have disrupted the DNA-replication cycle of Escherichia coli by inserting inverted Ter sites into the terminus region to delay completion of the chromosome. The inverted Ter sites (designated InvTer::spcr) were initially inserted into the chromosome of a delta tus strain to allow unrestrained chromosomal replication. We then introduced a functional tus gene by transforming the InvTer::spcr strain with a plasmid carrying the tus gene under control of an arabinose-inducible promoter. In the presence of 0.2% arabinose, the cells formed long filaments, suggesting that activation of the inverted Ter sites by Tus arrested DNA replication and delayed the onset of cell division. Induction of sfiA, a gene in the SOS regulon, was observed following arrest of DNA replication; however, when a sfiB114 allele was introduced into InvTer::spcr strain, long filaments were still formed, suggesting that the sfi-independent pathway also caused filamentation. Either recA::camr or lexA3 alleles suppressed filamentation when introduced in the InvTer strain. Interestingly, in both the recA::camr and lexA3 mutants, virtually all cells had a nucleoid, suggesting that cell div...Continue Reading

Citations

Aug 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Kuzminov
Dec 22, 1999·Current Opinion in Cell Biology·R B Jensen, L Shapiro
Mar 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Keiji Nagano, Hiroshi Nikaido
Mar 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Thomas EicherKlaas M Pos
Jun 2, 1997·The EMBO Journal·H BierneB Michel
Jan 15, 1997·The EMBO Journal·B MichelM Uzest
Jul 12, 2002·The EMBO Journal·Vladimir BidnenkoBénédicte Michel
Jul 12, 2013·The Journal of Antimicrobial Chemotherapy·Marius LinkeviciusDan I Andersson
Dec 12, 2012·Science·Abu Amar M Al MamunSusan M Rosenberg
Sep 22, 2001·Journal of Bacteriology·S Maisnier-PatinS Dasgupta
Sep 9, 2005·Microbiology and Molecular Biology Reviews : MMBR·Cameron NeylonNicholas E Dixon
Dec 19, 2007·PLoS Genetics·Emilie EsnaultFrédéric Boccard
Jan 2, 2013·Frontiers in Bioscience (Landmark Edition)·Chiara Indiani, Mike O'Donnell
Aug 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Danielle N MargalitDebabrata RayChaudhuri
Apr 8, 2000·Trends in Biochemical Sciences·B Michel
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·H I Zgurskaya, H Nikaido
Jul 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Matthew K HigginsVassilis Koronakis
May 23, 2014·Genome Biology and Evolution·David LaehnemannHinrich Schulenburg
Feb 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shirley YangE Lynn Zechiedrich
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Elena B TikhonovaHelen I Zgurskaya
Oct 22, 2005·Molecular Microbiology·Majda Valjavec-GratianThomas M Hill
Nov 21, 2008·Molecular Microbiology·Iain G DugginThomas M Hill
Apr 3, 2007·EMBO Reports·Karim Labib, Ben Hodgson
Jan 23, 2010·DNA Repair·Christian J RudolphRobert G Lloyd
Mar 25, 2011·Biochemical Society Transactions·Peter McGlynn
Dec 5, 2008·FEMS Microbiology Reviews·Jose Luis MartinezMaría Blanca Sánchez
Feb 12, 2009·FEMS Microbiology Reviews·Jose Luis MartinezCarolina Alvarez-Ortega
Oct 7, 2003·Annual Review of Microbiology·Jeffrey G Lawrence
Feb 3, 2007·FEMS Immunology and Medical Microbiology·Arinze S OkoliGeorge L Mendz
Aug 14, 1998·Molecular Microbiology·J P Bouché, S Pichoff
Sep 27, 1996·The Journal of Biological Chemistry·H BolhuisW N Konings
May 25, 2004·The Journal of Biological Chemistry·Elena B Tikhonova, Helen I Zgurskaya

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.