Insertion of mitochondrial DNA-encoded F1F0-ATPase subunit 8 across the mitochondrial inner membrane in vitro

The Journal of Biological Chemistry
M Ii, K Mihara

Abstract

Cytochrome oxidase subunits I, II, and III, the mitochondrial DNA-encoded proteins, are inserted across the inner membrane by the Oxa1p-containing translocator in a membrane potential-dependent manner. Oxa1p is also involved in the insertion of the cytoplasmically synthesized precursor of Oxa1p itself into the inner membrane from the matrix via the conservative sorting pathway. The mechanism of insertion of the other mitochondrially synthesized proteins, however, is unexplored. The insertion of the mitochondrial DNA-encoded subunit 8 of F(1)F(0)-ATPase (Su8) across the inner membrane was analyzed in vitro using the inverted inner membrane vesicles and the Escherichia coli lysate-synthesized substrate. This assay revealed that the N-terminal segment of Su8 inserted across the membrane to the intermembrane space and assumed the correct trans-cis topology depending on the mitochondrial matrix fraction. This translocation reaction was similar to those of Sec-independent, direct insertion pathways of E. coli and chloroplast thylakoid membranes. (i) It required neither nucleotide triphosphates nor membrane potential, and hydrophobic forces drove the process. (ii) It did not require protease-sensitive membrane components facing the ma...Continue Reading

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Citations

Dec 4, 2001·FEMS Microbiology Letters·M R YenM H Saier
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y Saint-GeorgesG Dujardin
Aug 16, 2005·Leukemia Research·Joli R WeissKirsten B Moysich
Sep 17, 2005·Progress in Nucleic Acid Research and Molecular Biology·Sharon H Ackerman, Alexander Tzagoloff
Apr 5, 2002·The Journal of Biological Chemistry·Frank BaumannJohannes M Herrmann

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