PMID: 7517394Jul 1, 1994Paper

Insertional activation of cepA leads to high-level beta-lactamase expression in Bacteroides fragilis clinical isolates

Journal of Bacteriology
M B RogersC J Smith

Abstract

Bacteroides fragilis is an important opportunistic pathogen of humans and is resistant to many drugs commonly used to treat anaerobic infections, including beta-lactams. A strain set comprised of B. fragilis isolates producing either low or high levels of the endogenous cephalosporinase activity, CepA, has been described previously (M. B. Rogers, A. C. Parker, and C. J. Smith, Antimicrob. Agents Chemother. 37:2391-2400, 1993). Clones containing cepA genes from each of seven representative strains were isolated, and the DNA sequences were determined. Nucleotide sequence comparisons revealed that there were few differences between the cepA coding sequences of the low- and high-activity strains. The cepA coding sequences were cloned into an expression vector, pFD340, and analyzed in a B. fragilis 638 cepA mutant. The results of beta-lactamase assays and ampicillin MICs showed that there was no significant difference in the enzymatic activity of structural genes from the high- or low-activity strains. Comparison of sequences upstream of the cepA coding region revealed that 50 bp prior to the translation start codon, the sequence for high-activity strains change dramatically. This region of the high-activity strains shared extensive...Continue Reading

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Citations

Sep 9, 2008·The Journal of Antimicrobial Chemotherapy·Nuria GarcíaAlberto Quesada
Nov 22, 2018·Frontiers in Genetics·Huizi TanWei Chen
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