Insertional assembly of actin filament barbed ends in association with formins produces piconewton forces

Proceedings of the National Academy of Sciences of the United States of America
David R Kovar, Thomas D Pollard

Abstract

Formins are large multidomain proteins required for assembly of actin cables that contribute to the polarity and division of animal and fungal cells. Formin homology-1 (FH1) domains bind profilin, and highly conserved FH2 domains nucleate actin filaments. We characterized the effects of two formins, budding yeast Bni1p and fission yeast Cdc12p, on actin assembly. We used evanescent wave fluorescence microscopy to observe assembly of actin filaments (i) nucleated by soluble formin FH1FH2 domains and (ii) associated with formin FH1FH2 domains immobilized on microscope slides. Bni1p(FH1FH2)p and Cdc12p(FH1FH2)p nucleated new actin filaments or captured the barbed ends of preformed actin filaments that grew by insertion of subunits between the immobilized formin and the barbed end of the filament. Both formins remained bound to growing actin filament barbed ends for >1,000 sec. Elongation of a filament between an immobilized formin and a second anchor point buckled filament segments as short as 0.7 microm, demonstrating that polymerization of single actin filaments produces forces of >1 piconewton, close to the theoretical maximum. After buckling, further growth produced long loops that did not supercoil, suggesting that formins do...Continue Reading

References

Dec 14, 2001·Proceedings of the National Academy of Sciences of the United States of America·K J Amann, T D Pollard
Jan 10, 2002·Biochemistry·Laurent Blanchoin, Thomas D Pollard
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Hyeong-Cheol Yang, Liza A Pon
Jul 23, 2002·Nature Cell Biology·Isabelle SagotDavid Pellman
Jan 15, 2003·Biochemistry·Martin PringSally H Zigmond
Mar 1, 2003·Biophysical Journal·Alex Mogilner, George Oster
Mar 26, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Marie-France CarlierDominique Pantaloni
May 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Paula A GiardiniJulie A Theriot
Jun 11, 2003·The Journal of Cell Biology·David R KovarThomas D Pollard
Jun 25, 2003·The Journal of Cell Biology·Marcel E JansonMarileen Dogterom
Jul 31, 2003·Trends in Cell Biology·Bradley J Wallar, Arthur S Alberts
Sep 19, 2003·Current Biology : CB·Arpita Upadhyaya, Alexander van Oudenaarden
Oct 17, 2003·Current Biology : CB·Sally H ZigmondMartin Pring
Mar 27, 2004·Science·Chiharu HigashidaNaoki Watanabe
Apr 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yann MarcyCécile Sykes
Jun 19, 2004·Current Biology : CB·Ian M SchwartzJames L McGrath

❮ Previous
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Citations

Aug 13, 2011·European Biophysics Journal : EBJ·Kinneret Keren
May 8, 2008·Journal of Mathematical Biology·Alex Mogilner
Jul 10, 2008·Journal of Mathematical Biology·Richard B Dickinson
Sep 21, 2013·Analytical Biochemistry·Maggie S Thomasson, Megan A Macnaughtan
Nov 21, 2013·European Journal of Cell Biology·Guillaume Romet-Lemonne, Antoine Jégou
Jun 14, 2005·Trends in Biochemical Sciences·Henry N Higgs
Jun 16, 2007·Biochemistry·Barbara WawroSarah E Hitchcock-DeGregori
May 12, 2010·Journal of the American Chemical Society·Syed Alipayam RizviSergey A Kozmin
Jan 28, 2005·Nature·Margot E QuinlanR Dyche Mullins
May 24, 2008·Nature·Kinneret KerenJulie A Theriot
Dec 2, 2004·Nature Cell Biology·David R Kovar, Thomas D Pollard
Oct 26, 2010·Nature Cell Biology·Cat M HaglundMatthew D Welch
May 23, 2013·Nature Communications·Antoine JégouGuillaume Romet-Lemonne
Dec 10, 2009·Nature Reviews. Molecular Cell Biology·Melissa A ChesaroneBruce L Goode
Aug 30, 2011·Nature Structural & Molecular Biology·Suk NamgoongRoberto Dominguez
Mar 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Syed Alipayam RizviSergey A Kozmin
Dec 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Charles I Fisher, Scot C Kuo
Jul 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Luis VidaliMagdalena Bezanilla
May 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Naomi CourtemancheEric C Greene
Apr 24, 2009·The Journal of Biological Chemistry·Kuo-Kuang Wen, Peter A Rubenstein
May 21, 2010·The Journal of Biological Chemistry·Christophe Le ClaincheMarie-France Carlier
Jan 17, 2012·The Journal of Biological Chemistry·Naomi Courtemanche, Thomas D Pollard
Jul 4, 2012·The Journal of Biological Chemistry·Lindsey E MalloyHeather L Bartlett
Jun 9, 2005·The Journal of Biological Chemistry·Melanie BarzikDorothy A Schafer
Feb 20, 2008·The Journal of Biological Chemistry·Lejla PasicDorothy A Schafer
Jun 26, 2008·The Journal of Biological Chemistry·Erin M NeidtDavid R Kovar
Mar 3, 2009·The Journal of Biological Chemistry·Aditya S Paul, Thomas D Pollard
Oct 16, 2013·The Journal of Cell Biology·Valerie C CoffmanJian-Qiu Wu
Oct 21, 2010·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Marc Herant, Micah Dembo
Mar 4, 2005·Molecular Biology of the Cell·David R KovarThomas D Pollard
Mar 9, 2007·Molecular Biology of the Cell·Shawnna M ButteryDavid Pellman
Aug 19, 2007·Molecular Biology of the Cell·Sophie G MartinFred Chang
Feb 29, 2008·Molecular Biology of the Cell·Ann YonetaniFred Chang
Feb 27, 2009·Molecular Biology of the Cell·Colleen T SkauDavid R Kovar
Aug 26, 2011·Molecular Biology of the Cell·Bonnie J ScottDavid R Kovar
Aug 25, 2012·Molecular Biology of the Cell·Hsin ChenDaniel J Lew
Apr 23, 2009·Human Molecular Genetics·Fen ZhouMarkus Dettenhofer
Oct 9, 2008·Molecular Biology and Evolution·Dimitra ChalkiaMasatoshi Nei
Nov 27, 2009·Plant Physiology·Agnieszka Esseling-OzdobaAnne Mie C Emons
Dec 8, 2009·Science·Thomas D Pollard, John A Cooper
Jan 8, 2011·Science·Thomas D Pollard
Oct 21, 2009·Infection and Immunity·Jason E HeindlMarcia B Goldberg

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