PMID: 7539418Jun 1, 1995Paper

Insertional inactivation of a chromosomal locus that modulates expression of potential virulence determinants in Staphylococcus aureus

Journal of Bacteriology
A L CheungA S Bayer

Abstract

A single insertion of transposon Tn551 into a unique chromosomal locus of Staphylococcus aureus ISP479C has resulted in a pleiotropic effect on the expression of both extracellular and cell wall proteins. In particular, the expression of cell wall protein A and clumping activity with fibrinogen were rendered undetectable in the mutant 1E3 compared with the parent. The secretion of alpha-hemolysin in mutant 1E3 was modestly increased. Southern blot and phenotypic analyses indicated that this locus is distinct from agr, xpr, and sar, three previously described global regulatory loci. Transduction experiments demonstrated that the genotype associated with mutant 1E3 could be transferred back into the parental strain ISP479C. The transductant 1E3-2 displayed a phenotypic profile similar to that of the original mutant. Northern (RNA) blot studies showed that this locus may be involved in modulating target genes at the mRNA level. In the rabbit endocarditis model, there was a significant decrease in both the infectivity rate and intravegetation bacterial density with mutant 1E3 compared with the parent at an inoculum of 10(3) CFU. Since protein A and the fibrinogen-binding protein(s) are major surface proteins that may mediate bacter...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1977·Infection and Immunity·P K PetersonP G Quie
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A L CheungV A Fischetti
Jun 1, 1990·The Journal of Infectious Diseases·A L Cheung, V A Fischetti
Jan 1, 1986·Molecular & General Genetics : MGG·P RecseiR P Novick
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B T NixonF M Ausubel
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R A LaddagaS Silver
Feb 1, 1994·Journal of Bacteriology·A L Cheung, P Ying
Nov 1, 1994·Analytical Biochemistry·A L CheungV A Fischetti
Nov 1, 1994·The Journal of Clinical Investigation·A L CheungA S Bayer

❮ Previous
Next ❯

Citations

Aug 1, 1996·FEMS Microbiology Letters·D Trivier, R J Courcol
Jun 7, 2003·Molecular Microbiology·S S IngavaleA L Cheung
Dec 20, 2003·Pharmacogenomics·Dezhong YinYinduo Ji
Jul 12, 2001·Infection and Immunity·K A SchmidtA L Cheung
Aug 23, 2003·Infection and Immunity·Katherine A SchmidtAmbrose L Cheung
Jun 12, 2002·Current Opinion in Microbiology·Dezhong Yin, Yinduo Ji

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.