PMID: 8602334Aug 1, 1995Paper

Insertional inactivation of binding determinants of Streptococcus crista CC5A using Tn916

Oral Microbiology and Immunology
F F CorreiaB Rosan

Abstract

Intermicrobial binding plays an important role in the ecology of the oral cavity because it represents one mechanism by which specific bacteria colonize dental plaque. The formation of "corncobs", a morphologically distinct microbial unit composed of Streptococcus crista and Fusobacterium nucleatum, is a highly specific binding interaction that depends on the presence of polar tufts of fimbriae on the streptococci. We have used a genetic approach to examine the role of streptococcal cell surface components involved in the binding of S. crista to F. nucleatum. Such binding may be an important component of corncob formation. A method for the genetic transformation of S. crista was used to transfer the broad host range transposon, Tn916, into the bacteria. Cells were grown to early log phase in brain heart infusion broth containing 10% fetal calf serum. The competent cells were mixed with purified DNA from pDL916, a plasmid construct consisting of Tn916 and the streptococcal/Escherichia coli shuttle vector pDL278. Over 300 transformants were screened for a reduction in binding to F. nucleatum. Five of the transformants showed a change in binding ranging from 59% to 29% of the positive control values. Southern blots revealed that t...Continue Reading

References

Oct 1, 1991·International Journal of Systematic Bacteriology·P HandleyR A Whiley
Apr 1, 1991·Applied and Environmental Microbiology·G M DunnyD J LeBlanc
Jun 1, 1990·Infection and Immunity·R J Lamont, B Rosan
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M G Caparon, J R Scott
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J R ScottM G Caparon
Jun 1, 1970·Journal of Bacteriology·J W Lawson, H Gooder
Apr 1, 1983·Infection and Immunity·P LancyS C Holt
Aug 1, 1980·Infection and Immunity·P LancyB Rosan
Jan 1, 1981·Annual Review of Biochemistry·H O SmithR A Deich
Oct 1, 1993·Oral Microbiology and Immunology·R J LamontB Rosan
Sep 1, 1993·Journal of Bacteriology·D J Harrington, R R Russell
Mar 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·A TOMASZ, R D HOTCHKISS

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.