PMID: 7541063Jul 1, 1995Paper

Insertional sequence primers for Bordetella pertussis diagnostic polymerase chain reaction differentiate strains of Pseudomonas cepacia

The Journal of Infectious Diseases
N Cimolai, C Trombley

Abstract

Insertion sequence primers originally intended to amplify a singular specific product for the rapid diagnosis of Bordetella pertussis respiratory infection were used to differentiate strains of Pseudomonas (Burkholderia) cepacia. A modified sample preparation of proteinase K treatment and boiling was used in lieu of DNA extraction. The method was simple, rapid, and reproducible. This scheme identified 10 variations among 35 strains. Repeat strains from patients with cystic fibrosis and epidemiologically linked strains from an infection associated with a jet gun injection device were homologous in each setting.

Citations

Dec 1, 1996·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·B ZuckerF Horsch
Aug 1, 1996·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Nevio Cimolai, Colleen Trombley
Jun 2, 2001·Clinical Rehabilitation·S J PageM V Johnston
Jun 17, 1998·FEMS Microbiology Letters·R BorrowA J Fox
Dec 31, 2002·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·F Doucet-PopulaireP Foucaud
Apr 1, 1996·Journal of Clinical Microbiology·E ReizensteinH O Hallander

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