Insertions of a novel class of transposable elements with a strong target site preference at the r locus of maize.

Genetics
E L WalkerS L Dellaporta

Abstract

The r locus of maize regulates anthocyanin synthesis in various tissues of maize through the production of helix-loop-helix DNA binding proteins capable of inducing expression of structural genes in the anthocyanin biosynthetic pathway. The complex r variant, R-r: standard (R.r), undergoes frequent mutation through a variety of mechanisms including displaced synapsis and crossing over, and intrachromosomal recombination. Here we report a new mechanism for mutation at the R-r complex: insertion of a novel family of transposable elements. Because the elements were first identified in the R-p gene of the R-r complex, they have been named P instability Factor (PIF). Two different PIF elements were cloned and found to have identical sequences at their termini but divergent internal sequences. In addition, the PIF elements showed a marked specificity of insertion sites. Six out of seven PIF-containing derivatives examined had an element inserted at an identical location. Two different members of the PIF element family were identified at this position. The seventh PIF-containing derivative examined had the element inserted at a distinct position within r. Even at this location, however, the element inserted into a conserved target seq...Continue Reading

Citations

Mar 14, 2000·Current Opinion in Plant Biology·V Walbot
Jul 28, 2012·Plant Physiology·Benjamin HartwigFranziska Turck
Jun 11, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·E AlvesA M Vázquez
Apr 22, 2006·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Ahu AltinkutAlexander Belyayev
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Apr 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xiaoyu Zhang, Susan R Wessler
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·Q ZhangS R Wessler

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