Insight into bovine (Bos indicus) spermatozoal whole transcriptome profile

Theriogenology
Nidhi P RavalChaitanya G Joshi

Abstract

Mature spermatozoa harbor both coding and non-coding type of RNAs which regulates spermatogenesis, fertilization and early development. Characterization of bovine sperm transcriptome can provide more insight into the molecular mechanisms involved in these processes. Here, we have analyzed whole transcriptome profile of Bos indicus spermatozoa to access the global RNA expression. RNA-Seq analysis identified 14,306 genes expressed with FPKM >0, while 405 genes expressed when threshold increased to FPKM >5. Functional annotations showed that sperm transcripts were associated with molecular processes (translation, ribosomal small and large subunit assembly) and cellular components (cytosolic small and large ribosomal subunit and membranes) related to known sperm functions at fertilization and spermatogenesis. The RNA-Seq data was validated using droplet digital PCR where both highly abundant gene viz. RN7SL1 and less abundant gene viz. ZFP280B were validated. This study may provide future directions in reproductive biology of Bos indicus.

References

Jan 1, 1991·Progress in Nucleic Acid Research and Molecular Biology·Rafael Oliva, G H Dixon
Mar 9, 1973·Nature·C J Betlach, R P Erickson
May 24, 1974·Nature·H O Besedovsky, E Sorkin
Oct 23, 1997·International Review of Cytology·S B McLeskeyP M Saling
May 7, 1998·The International Journal of Biochemistry & Cell Biology·D S Latchman
May 20, 1998·Genome Research·N KenmochiD C Page
Dec 10, 1999·Nature·P SutovskyG Schatten
Feb 24, 2001·Molecular Aspects of Medicine·P T LieuY Yang
Sep 21, 2002·Lancet·G Charles OstermeierStephen A Krawetz
Apr 3, 2003·Microscopy Research and Technique·Jean-Pierre Dadoune
Sep 2, 2005·Nature Reviews. Genetics·Stephen A Krawetz
Apr 4, 2006·Human Reproduction Update·Rafael Oliva
Jul 20, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Isabelle GilbertClaude Robert
Aug 23, 2007·Asian Journal of Andrology·Isabelle Galeraud-DenisSerge Carreau
Mar 29, 2008·Science·Paulo P AmaralJohn S Mattick
Jul 20, 2010·Reproductive Biomedicine Online·J M FeugangE Memili
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rui SongWei Yan
Mar 9, 2013·Nucleic Acids Research·Edward SendlerStephen A Krawetz
Apr 11, 2013·PloS One·Matthew C StrainDouglas D Richman
Jul 17, 2013·Human Reproduction Update·Meritxell JodarReproductive Medicine Network
Apr 10, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Alfred Chun-Shui LukTin-Lap Lee
Aug 13, 2014·BMC Genomics·Marc SultanMarie-Laure Yaspo
Sep 24, 2014·Journal of Assisted Reproduction and Genetics·Eva TvrdaAshok Agarwal
Jul 11, 2015·Science Translational Medicine·Meritxell JodarStephen A Krawetz
Aug 1, 2015·Cell and Tissue Research·Meritxell JodarStephen A Krawetz
Feb 13, 2016·Genome Génome / Conseil National De Recherches Canada·R P SinghP A Azeez
Mar 10, 2017·Scientific Reports·Sellappan SelvarajuJanivara Parameshwaraiah Ravindra
Aug 29, 2017·The Journal of Reproduction and Development·Xiaoxia RenDong Wang

Citations

May 27, 2021·Frontiers in Cell and Developmental Biology·Mani Arul PrakashTirtha Kumar Datta

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