Insight into bovine (Bos indicus) spermatozoal whole transcriptome profile

Nidhi P RavalChaitanya G Joshi


Mature spermatozoa harbor both coding and non-coding type of RNAs which regulates spermatogenesis, fertilization and early development. Characterization of bovine sperm transcriptome can provide more insight into the molecular mechanisms involved in these processes. Here, we have analyzed whole transcriptome profile of Bos indicus spermatozoa to access the global RNA expression. RNA-Seq analysis identified 14,306 genes expressed with FPKM >0, while 405 genes expressed when threshold increased to FPKM >5. Functional annotations showed that sperm transcripts were associated with molecular processes (translation, ribosomal small and large subunit assembly) and cellular components (cytosolic small and large ribosomal subunit and membranes) related to known sperm functions at fertilization and spermatogenesis. The RNA-Seq data was validated using droplet digital PCR where both highly abundant gene viz. RN7SL1 and less abundant gene viz. ZFP280B were validated. This study may provide future directions in reproductive biology of Bos indicus.


Jan 1, 1991·Progress in Nucleic Acid Research and Molecular Biology·Rafael Oliva, G H Dixon
Mar 9, 1973·Nature·C J Betlach, R P Erickson
May 24, 1974·Nature·H O Besedovsky, E Sorkin
Oct 23, 1997·International Review of Cytology·S B McLeskeyP M Saling
May 7, 1998·The International Journal of Biochemistry & Cell Biology·D S Latchman
May 20, 1998·Genome Research·N KenmochiD C Page
Dec 10, 1999·Nature·P SutovskyG Schatten
Feb 24, 2001·Molecular Aspects of Medicine·P T LieuY Yang
Sep 21, 2002·Lancet·G Charles OstermeierStephen A Krawetz
Apr 3, 2003·Microscopy Research and Technique·Jean-Pierre Dadoune
Sep 2, 2005·Nature Reviews. Genetics·Stephen A Krawetz
Apr 4, 2006·Human Reproduction Update·Rafael Oliva
Jul 20, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Isabelle GilbertClaude Robert
Aug 23, 2007·Asian Journal of Andrology·Isabelle Galeraud-DenisSerge Carreau
Mar 29, 2008·Science·Paulo P AmaralJohn S Mattick
Jul 20, 2010·Reproductive Biomedicine Online·J M FeugangE Memili
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rui SongWei Yan
Mar 9, 2013·Nucleic Acids Research·Edward SendlerStephen A Krawetz
Apr 11, 2013·PloS One·Matthew C StrainDouglas D Richman
Jul 17, 2013·Human Reproduction Update·Meritxell JodarReproductive Medicine Network
Apr 10, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Alfred Chun-Shui LukTin-Lap Lee
Aug 13, 2014·BMC Genomics·Marc SultanMarie-Laure Yaspo
Sep 24, 2014·Journal of Assisted Reproduction and Genetics·Eva TvrdaAshok Agarwal
Jul 11, 2015·Science Translational Medicine·Meritxell JodarStephen A Krawetz
Aug 1, 2015·Cell and Tissue Research·Meritxell JodarStephen A Krawetz
Feb 13, 2016·Genome Génome / Conseil National De Recherches Canada·R P SinghP A Azeez
Mar 10, 2017·Scientific Reports·Sellappan SelvarajuJanivara Parameshwaraiah Ravindra
Aug 29, 2017·The Journal of Reproduction and Development·Xiaoxia RenDong Wang


May 27, 2021·Frontiers in Cell and Developmental Biology·Mani Arul PrakashTirtha Kumar Datta

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.