PMID: 9189008Jan 1, 1997Paper

Insight into screening immunoglobulin gene combinatorial libraries in a phage display vector: a tale of two antibodies

Autoimmunity
A KakinumaSandra M McLachlan

Abstract

Combinatorial libraries of immunoglobulin genes in "phage display" vectors are a powerful tool for obtaining antigen-specific antibody fragments. To date, this approach has been used to isolate abundant, but not rare, human autoantibodies of IgG class. We have compared the relative efficiencies of panning pComb3 libraries made from intrathyroidal plasma cells for abundant human autoantibodies to thyroid peroxidase (TPO) and rare autoantibodies to the thyrotropin receptor (TSHR). TPO-specific Fab were readily obtained from a library using three different forms of recombinant antigen, (i) purified TPO, (ii) impure TPO in culture medium and, (iii) TPO expressed on the surface of CHO cells. In contrast, TSHR-specific Fab were not isolated. This was the case despite repeated pannings of six libraries from three optimal patients (IgG/kappa and IgG/lambda libraries for each patient). Both purified recombinant TSHR and CHO cells expressing TSHR on their surface were used. Library enrichment was observed on some screenings. However, Fab expressed by individual clones or from enriched libraries were not specific as determined by (i) binding to purified, radio-labeled antigen, (ii) FACS analysis of TSHR on intact CHO cells and, (iii) inhi...Continue Reading

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Citations

Jan 10, 2003·The Journal of Clinical Endocrinology and Metabolism·Francesco LatrofaSandra M McLachlan
Apr 8, 2010·Current Protocols in Neuroscience·Andrew R M Bradbury
Oct 3, 2021·Journal for Immunotherapy of Cancer·Huicheng LiuXiang-Ping Yang

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