Insight into the assembly properties and functional organisation of the magnetotactic bacterial actin-like homolog, MamK.

PloS One
Sanjiv SonkariaDamien Faivre

Abstract

Magnetotactic bacteria (MTB) synthesize magnetosomes, which are intracellular vesicles comprising a magnetic particle. A series of magnetosomes arrange themselves in chains to form a magnetic dipole that enables the cell to orient itself along the Earth's magnetic field. MamK, an actin-like homolog of MreB has been identified as a central component in this organisation. Gene deletion, fluorescence microscopy and in vitro studies have yielded mechanistic differences in the filament assembly of MamK with other bacterial cytoskeletal proteins within the cell. With little or no information on the structural and behavioural characteristics of MamK outside the cell, the mamK gene from Magnetospirillium gryphiswaldense was cloned and expressed to better understand the differences in the cytoskeletal properties with its bacterial homologues MreB and acitin. Despite the low sequence identity shared between MamK and MreB (22%) and actin (18%), the behaviour of MamK monitored by light scattering broadly mirrored that of its bacterial cousin MreB primarily in terms of its pH, salt, divalent metal-ion and temperature dependency. The broad size variability of MamK filaments revealed by light scattering studies was supported by transmission e...Continue Reading

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Citations

Oct 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Hyeran KangEnrique M De La Cruz
Dec 4, 2012·The Journal of Biological Chemistry·Ertan OzyamakArash Komeili
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Datasets Mentioned

BETA
CAE12034.1

Methods Mentioned

BETA
circular dichroism
light scattering
Dynamic light scattering
GTPase
small angle scattering
electrophoresis

Software Mentioned

BLAST
MamK
PSI
zeta sizer
Firestar
Adaptive Poisson - Boltzmann Solver ( APBS )
ParM
Image J
Dispersion Technology
Modeller

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