Insight into the binding of antifreeze proteins to ice surfaces via 13C spin lattice relaxation solid-state NMR.

Biophysical Journal
Yougang Mao, Yong Ba

Abstract

The primary sequences of type I antifreeze proteins (AFPs) are Ala rich and contain three 11-residue repeat units beginning with threonine residues. Their secondary structures consist of alpha-helices. Previous activity study of side-chain mutated AFPs suggests that the ice-binding side of type I AFPs comprises the Thr side chains and the conserved i + 4 and i + 8 Ala residues, where i indicates the positions of the Thrs. To find structural evidence for the AFP's ice-binding side, a variable-temperature dependent (13)C spin lattice relaxation solid-state NMR experiment was carried out for two Ala side chain (13)C labeled HPLC6 isoforms of the type I AFPs each frozen in H(2)O and D(2)O, respectively. The first one was labeled on the equivalent 17th and 21st Ala side chains (i + 4, 8), and the second one on the equivalent 8th, 19th, and 30th Ala side chains (i + 6). The two kinds of labels are on the opposite sides of the alpha-helical AFP. A model of Ala methyl group rotation/three-site rotational jump combined with water molecular reorientation was tested to probe the interactions of the methyl groups with the proximate water molecules. Analysis of the T(1) data shows that there could be 10 water molecules closely capping an i ...Continue Reading

References

Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J A Raymond, A L DeVries
Dec 18, 1991·European Journal of Biochemistry·A Chakrabartty, C L Hew
Mar 7, 1969·Science·A L DeVries, D E Wohlschlag
Dec 16, 1997·Biochemistry·H ChaoF D Sönnichsen
Jan 29, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·F D SönnichsenB D Sykes
Sep 22, 1999·European Journal of Biochemistry·M M HardingA D Haymet
Dec 22, 1999·FEBS Letters·J BaardsnesP L Davies
Feb 22, 2001·Annual Review of Physiology·J G Duman

❮ Previous
Next ❯

Citations

Oct 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ansgar B SiemerAnn E McDermott
Jan 1, 2011·Case Reports in Infectious Diseases·M T García-RomeroE Cervera-Ceballos
Nov 29, 2011·International Journal of Microbiology·Giulia Morace, Elisa Borghi
Dec 17, 2014·The Journal of Chemical Physics·Ivan V SergeyevAnn E McDermott
Dec 6, 2014·Extremophiles : Life Under Extreme Conditions·Charles V RiceRavindranth Garimella
Mar 19, 2015·Physical Chemistry Chemical Physics : PCCP·S Alireza BagherzadehPeter Englezos
Mar 1, 2018·European Biophysics Journal : EBJ·Antonia FloresYong Ba
Oct 20, 2019·International Journal of Molecular Sciences·Romà Surís-Valls, Ilja K Voets
Sep 18, 2010·Colloids and Surfaces. B, Biointerfaces·Osnat Younes-MetzlerJavier B Giorgi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.