Insight into the interactions of proteinase inhibitor- alpha-2-macroglobulin with hypochlorite

International Journal of Biological Macromolecules
Tooba SiddiquiFahim Halim Khan

Abstract

Hypochlorous acid (HOCl), an active bleaching agent is one of the major oxidant produced by neutrophils under physiological conditions. It is among one of the most potent reactive oxygen species (ROS) which causes oxidation of biomolecules. Treatment of proteins with hypochlorite results in direct oxidative damage to the protein. Alpha-2-macroglobulin (α2M) is a major proteinase inhibitor that can inhibit proteinase of any kind regardless of their specificity and catalytic mechanism. The proteinase-antiproteinase balance plays an important role in mediating inflammation associated tissue destruction. In this paper, we intend to study hypochlorite induced modifications in proteinase inhibitor- α2M via biophysical techniques such as absorption spectroscopy, fluorescence spectroscopy, circular dichroism (CD), fourier transform infrared spectrometry (FTIR) and isothermal titration calorimetry (ITC). It was found that hypochlorite decreases the anti-proteolytic potential and causes inactivation of sheep α2M. It also causes structural and functional change in sheep α2M as evident by UV-Visible absorption spectroscopy and fluorescence measurements. Change in secondary structure of α2M was confirmed by CD and FTIR. Thermodynamics param...Continue Reading

Citations

Sep 24, 2019·Journal of Biomolecular Structure & Dynamics·Tooba SiddiquiFahim Halim Khan
Aug 21, 2019·Oxidative Medicine and Cellular Longevity·Jordan H CaterAmy R Wyatt
Jan 22, 2020·Journal of Food Biochemistry·Syed Saqib AliFahim Halim Khan
Jul 22, 2019·Journal of Fluorescence·Tooba SiddiquiFahim Halim Khan
Jun 21, 2020·Biomolecules·Alexandra VasilyevaMark Rosenfeld
Aug 3, 2021·Biochimica Et Biophysica Acta. General Subjects·Mark A RosenfeldLyubov V Yurina

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