Insight into the Salivary Gland Transcriptome of Lygus lineolaris (Palisot de Beauvois)

PloS One
Kurt C ShowmakerNatraj Krishnan

Abstract

The tarnished plant bug (TPB), Lygus lineolaris (Palisot de Beauvois) is a polyphagous, phytophagous insect that has emerged as a major pest of cotton, alfalfa, fruits, and vegetable crops in the eastern United States and Canada. Using its piercing-sucking mouthparts, TPB employs a "lacerate and flush" feeding strategy in which saliva injected into plant tissue degrades cell wall components and lyses cells whose contents are subsequently imbibed by the TPB. It is known that a major component of TPB saliva is the polygalacturonase enzymes that degrade the pectin in the cell walls. However, not much is known about the other components of the saliva of this important pest. In this study, we explored the salivary gland transcriptome of TPB using Illumina sequencing. After in silico conversion of RNA sequences into corresponding polypeptides, 25,767 putative proteins were discovered. Of these, 19,540 (78.83%) showed significant similarity to known proteins in the either the NCBI nr or Uniprot databases. Gene ontology (GO) terms were assigned to 7,512 proteins, and 791 proteins in the sialotranscriptome of TPB were found to collectively map to 107 Kyoto Encyclopedia of Genes and Genomes (KEGG) database pathways. A total of 3,653 Pfam...Continue Reading

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Citations

Nov 23, 2017·Scientific Reports·Yong ZhangJulian Chen
Aug 5, 2017·Standards in Genomic Sciences·Kurt C ShowmakerShi-En Lu
Apr 1, 2018·Current Opinion in Insect Science·Kristen A Panfilio, David R Angelini
May 12, 2021·Journal of Insect Science·Omaththage P PereraGordon L Snodgrass
Aug 22, 2020·Current Opinion in Insect Science·Pablo Emiliano Cantón, Bryony C Bonning

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Datasets Mentioned

BETA
ABD63920
PRJNA280549
GDAW00000000

Methods Mentioned

BETA
electrophoresis
RNA-Seq

Software Mentioned

InterProScan
SUPERFAMILY
MEGA
Trimmomatic
BLASTP
PrositeProfiles
ClustalW
Prosite
Bowtie2
PIRSF

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