Insight into vitronectin structural evolution on material surface chemistries: The mediation for cell adhesion

Bioactive Materials
Tianjie LiYingjun Wang

Abstract

Biomaterial surface chemistry engenders profound consequences on cell adhesion and the ultimate tissue response by adsorbing proteins from extracellular matrix, where vitronectin (Vn) is involved as one of the crucial mediator proteins. Deciphering the adsorption behaviors of Vn in molecular scale provides a useful account of how to design biomaterial surfaces. But the details of structural dynamics and consequential biological effect remain elusive. Herein, both experimental and computational approaches were applied to delineate the conformational and orientational evolution of Vn during adsorption onto self-assembled monolayers (SAMs) terminating with -COOH, -NH2, -CH3 and -OH. To unravel the interplay between cell binding and the charge and wettability of material surface, somatomedin-B (SMB) domain of Vn holding the RGD cell-binding motif was employed in molecular dynamics (MD) simulations, with orientation initialized by Monte Carlo (MC) method. Experimental evidences including protein adsorption, cell adhesion and integrin gene expressions were thoroughly investigated. The adsorption of Vn on different surface chemistries showed very complex profiles. Cell adhesion was enabled on all Vn-adsorbed surfaces but with distinct...Continue Reading

Citations

Sep 5, 2020·Critical Reviews in Food Science and Nutrition·Xin LuXiulan Sun
Apr 20, 2021·Bioactive Materials·Seung-Hoon UmHojeong Jeon
May 10, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Andreia CerqueiraIsabel Goñi
Jul 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Deepthi SankarJayakumar Rangasamy

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Methods Mentioned

BETA
X-ray
surface plasmon resonance
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

VMD
PDB2PQR
mmpbsa
dssp package
APBS
CHARMM36
GROMACS
PyMOL

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