Insights from a new analytical electrophoresis apparatus

Journal of Pharmaceutical Sciences
T M LaueD A Stevenson

Abstract

Charge is a fundamental property of macromolecules that is inextricably linked to their structure, solubility, stability, and interactions. Progress has been made on the theoretical and structural aspects of these relationships. However, for several reasons, charge is difficult to measure in solution. Consequently, there is a lack of experimental data that, independent of other macro-ion properties, determines the effective charge. To overcome this problem, novel instrumentation and methods are being developed in our laboratory. Described here is an analytical electrophoresis apparatus that permits both the measurement of electrophoretic mobilities and the determination of steady-state electrophoresis concentration distributions. The latter provides a different-perspective on the processes that influence macro-ion behavior in an electric field. In addition, the apparatus permits the determination of diffusion coefficients either from boundary spreading during transport or from the decay of a concentration gradient. All of these determinations can be made with a single, 8-microL sample in a variety of solvents, thus providing unique insights into the charge properties of a macro-ion. Presented here is a progress report about thi...Continue Reading

References

Nov 1, 1989·Analytical Biochemistry·T M LaueD A Yphantis
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J E Godfrey
Nov 30, 1945·Annals of the New York Academy of Sciences·H S HARNED, D M FRENCH

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Citations

Mar 27, 2004·Biophysical Chemistry·Thomas P Moody, Harvey K Shepard
Jan 24, 2002·Biophysical Chemistry·S A Allison
Dec 19, 2002·Biophysical Chemistry·Jennifer A DurantStuart A Allison
May 4, 2002·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Anna-Kaisa KontturiArto Urtti
Jul 17, 1998·Current Opinion in Structural Biology·H H StreyV A Parsegian
Dec 3, 2008·The Journal of Chemical Physics·O Gonzalez, J Li
May 8, 1999·Biophysical Journal·S A AllisonJ O Wooll
Mar 23, 2011·Electrophoresis·Mònica MirJosep Samitier
Aug 27, 1998·Electrophoresis·T M RidgewayT M Laue
Feb 25, 2010·Chemical Society Reviews·Ulrich F KeyserSerge G Lemay

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