Insights from molecular signature of in vivo cardiac c-Kit(+) cells following cardiac injury and β-catenin inhibition

Journal of Molecular and Cellular Cardiology
Conrad P HodgkinsonVictor J Dzau

Abstract

There is much interest over resident c-Kit(+) cells in tissue regeneration. Their role in cardiac regeneration has been controversial. In this study we aim to understand the in vivo behavior of cardiac c-Kit(+) cells at baseline and after myocardial infarction and in response to Sfrp2. This approach can accurately study the in vivo transcript expressions of these cells in temporal response to injury and overcomes the limitations of the in vitro approach. RNA-seq was performed with c-Kit(+) cells and cardiomyocytes from healthy non-injured mice as well as c-Kit(+) cells from 1 day post-MI and 12 days post-MI mice. When compared to in vivo c-Kit(+) cells isolated from a healthy non-injured mouse heart, cardiomyocytes were enriched in transcripts that express anion channels, cation channels, developmental/differentiation pathway components, as well as proteins that inhibit canonical Wnt/β-catenin signaling. Myocardial infarction (MI) induced in vivo c-Kit(+) cells to transiently adopt the cardiomyocyte-specific signature: expression of a number of cardiomyocyte-specific transcripts was maximal 1 day post-MI and declined by 12 days post-MI. We next studied the effect of β-catenin inhibition on in vivo c-Kit(+) cells by administerin...Continue Reading

References

Feb 13, 2001·Genes & Development·V A Schneider, M Mercola
Feb 13, 2001·Genes & Development·M J MarvinA B Lassar
Apr 5, 2001·Nature·D OrlicP Anversa
Sep 25, 2003·Cell·Antonio P BeltramiPiero Anversa
Dec 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Atsuhiko T NaitoIssei Komuro
Jul 17, 2007·Progress in Cardiovascular Diseases·Lucio BarileEduardo Marbán
Jul 24, 2010·Circulation Research·Susanne Gessert, Michael Kühl
Oct 18, 2012·Physiological Reviews·Johan Lennartsson, Lars Rönnstrand
Jan 1, 2013·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Marimuthu Saravanakumar, Halagowder Devaraj
Mar 7, 2013·Circulation Research·Devaveena DeyJoseph C Wu
May 9, 2014·Nature·Jop H van BerloJeffery D Molkentin
Jun 7, 2014·Current Treatment Options in Cardiovascular Medicine·Kyung U Hong, Roberto Bolli
Jul 22, 2014·The Journal of Comparative Neurology·Bradley J GoldsteinJoshua M Hare
Oct 10, 2014·Circulation Research·Conrad P HodgkinsonVictor J Dzau
Jun 15, 2015·Journal of Molecular and Cellular Cardiology·Jeffrey SchmeckpeperMaria Mirotsou
Oct 31, 2015·Nature Communications·Nishat SultanaChen-Leng Cai
Dec 5, 2015·Cell Research·Qiaozhen LiuBin Zhou
Dec 15, 2015·Scientific Reports·Yiqiang ZhangCharles Wang
Oct 30, 2016·Circulation Research·Annarosa Leri, Piero Anversa
Nov 12, 2016·Circulation·Tamer M A MohamedDeepak Srivastava
Jul 8, 2017·Circulation·Thomas EschenhagenJoseph A Hill
Nov 14, 2017·Nature Medicine·Lingjuan HeBin Zhou
Mar 23, 2018·Nature·Carla VicinanzaDaniele Torella
Apr 11, 2018·Circulation Research·Natalie A GudeMark A Sussman

❮ Previous
Next ❯

Citations

Jul 6, 2019·Frontiers in Endocrinology·Fabiola MarinoDaniele Torella
Jan 27, 2021·Cells·Evangelos P Daskalopoulos, W Matthijs Blankesteijn
Feb 16, 2021·Physiological Reports·Ying-Chang HsuehJose A Gomez
Mar 20, 2021·Stem Cell Research & Therapy·Mika J VälimäkiHeikki Ruskoaho
Aug 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·José A GomezVictor J Dzau

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cardiotoxicity

Cardiotoxicity refers to the muscle damage or dysfunction of heart electrophysiology caused by drug intake or due to disease complications. It is a well-known side effect of several cytotoxic drugs, especially of the anthracyclines and can lead to long term morbidity. Here is the latest research.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.