Insights from the crystal structure of the sixth BRCT domain of topoisomerase IIbeta binding protein 1.

Protein Science : a Publication of the Protein Society
Charles Chung Yun LeungJ N Mark Glover

Abstract

Topoisomerase IIbeta binding protein 1 (TopBP1) is a major player in the DNA damage response and interacts with a number of protein partners via its eight BRCA1 carboxy-terminal (BRCT) domains. In particular, the sixth BRCT domain of TopBP1 has been implicated in binding to the phosphorylated transcription factor, E2F1, and poly(ADP-ribose) polymerase 1 (PARP-1), where the latter interaction is responsible for the poly(ADP-ribosyl)ation of TopBP1. To gain a better understanding of the nature of TopBP1 BRCT6 interactions, we solved the crystal structure of BRCT6 to 1.34 A. The crystal structure reveals a degenerate phospho-peptide binding pocket and lacks conserved hydrophobic residues involved in packing of tandem BRCT repeats, which, together with results from phospho-peptide binding studies, strongly suggest that TopBP1 BRCT6 independently does not function as a phospho-peptide binding domain. We further provide insight into poly(ADP-ribose) binding and sites of potential modification by PARP-1.

Citations

Dec 4, 2010·The Journal of Biological Chemistry·Charles Chung Yun LeungJ N Mark Glover
Aug 21, 2010·Nucleic Acids Research·Mathieu RappasLaurence H Pearl
Oct 5, 2011·BMC Structural Biology·Paul A LoefflerRobert E London
Aug 5, 2014·DNA Repair·Christopher P WardlawAntony W Oliver
Feb 24, 2015·Progress in Biophysics and Molecular Biology·Qian WuTom L Blundell

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