Insights in the Antimicrobial Potential of the Natural Nisin Variant Nisin H

Frontiers in Microbiology
Jens ReinersLutz Schmitt

Abstract

Lantibiotics are a growing class of antimicrobial peptides, which possess antimicrobial activity against mainly Gram-positive bacteria including the highly resistant strains such as methicillin-resistant Staphylococcus aureus or vancomycin-resistant enterococci. In the last decades numerous lantibiotics were discovered in natural habitats or designed with bioengineering tools. In this study, we present an insight in the antimicrobial potential of the natural occurring lantibiotic nisin H from Streptococcus hyointestinalis as well as the variant nisin H F1I. We determined the yield of the heterologously expressed peptide and quantified the cleavage efficiency employing the nisin protease NisP. Furthermore, we analyzed the effect on the modification via mass spectrometry analysis. With standardized growth inhibition assays we benchmarked the activity of pure nisin H and the variant nisin H F1I, and their influence on the activity of the nisin immunity proteins NisI and NisFEG from Lactococcus lactis and the nisin resistance proteins SaNSR and SaNsrFP from Streptococcus agalactiae COH1. We further checked the antibacterial activity against clinical isolates of Staphylococcus aureus, Enterococcus faecium and Enterococcus faecalis v...Continue Reading

References

Nov 1, 1991·European Journal of Biochemistry·J W MuldersW M de Vos
May 24, 1967·Journal of the American Chemical Society·E Gross, J L Morell
Aug 1, 1995·Applied and Environmental Microbiology·H S RollemaR J Siezen
Feb 1, 1996·Antonie van Leeuwenhoek·J Delves-BroughtonJ Hugenholtz
Apr 1, 1997·Trends in Biotechnology·O P KuipersW M de Vos
Jun 1, 1997·Applied and Environmental Microbiology·B L RothP J Millard
Jun 12, 1998·Biochemistry·E BreukinkO P Kuipers
Apr 24, 1999·European Journal of Biochemistry·A Karakas SenM J Gasson
Dec 22, 1999·Antimicrobial Agents and Chemotherapy·M Mota-MeiraM C Lavoie
Oct 3, 2002·Biochemistry·Hester Emilie van HeusdenEefjan Breukink
Nov 19, 2003·Biochemistry·Nicole M OkeleyWilfred A van der Donk
May 6, 2004·Journal of Bacteriology·Imke WiedemannHans-Georg Sahl
Sep 8, 2004·Biochemistry·Hester Emilie HasperEefjan Breukink
Sep 14, 2004·Nature Structural & Molecular Biology·Shang-Te D HsuNico A J van Nuland
Aug 10, 2005·Applied Microbiology and Biotechnology·Igor Mierau, Michiel Kleerebezem
Dec 1, 1993·Applied and Environmental Microbiology·P R Jensen, K Hammer
Dec 29, 2005·Biotechnology Advances·Xu Xia ZhouYuan Jiang Pan
Feb 8, 2006·Applied and Environmental Microbiology·Ruth E WirawanJohn R Tagg
Oct 1, 1928·Journal of Bacteriology·L A Rogers, E O Whittier
May 22, 2007·The Journal of Biological Chemistry·Bo Li, Wilfred A van der Donk
Jul 22, 2009·The Journal of Biological Chemistry·Jacek LubelskiOscar P Kuipers
Nov 26, 2010·Applied and Environmental Microbiology·Annechien PlatGert N Moll
Jan 12, 2011·Antimicrobial Agents and Chemotherapy·Daniela JabésStefano Donadio
Jul 11, 2012·Current Opinion in Pharmacology·Michael John Dawson, Richard W Scott
Sep 12, 2012·The Journal of Antimicrobial Chemotherapy·Grace S CrowtherMark H Wilcox
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Qi ZhangWilfred A van der Donk
Jul 23, 2013·Biochemistry·André AbtsLutz Schmitt
Nov 12, 2013·International Journal of Medical Microbiology : IJMM·Jasmin DischingerGabriele Bierbaum
Jul 12, 2014·PloS One·Zainab AlKhatibSander H J Smits
Apr 5, 2015·Applied and Environmental Microbiology·Paula M O'ConnorColin Hill
May 8, 2015·Nature Protocols·Lawrence A KelleyMichael J E Sternberg

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