Insights into bacterial lipoprotein trafficking from a structure of LolA bound to the LolC periplasmic domain.

Proceedings of the National Academy of Sciences of the United States of America
Elise KaplanVassilis Koronakis

Abstract

In Gram-negative bacteria, outer-membrane lipoproteins are essential for maintaining cellular integrity, transporting nutrients, establishing infections, and promoting the formation of biofilms. The LolCDE ABC transporter, LolA chaperone, and LolB outer-membrane receptor form an essential system for transporting newly matured lipoproteins from the outer leaflet of the cytoplasmic membrane to the innermost leaflet of the outer membrane. Here, we present a crystal structure of LolA in complex with the periplasmic domain of LolC. The structure reveals how a solvent-exposed β-hairpin loop (termed the "Hook") and trio of surface residues (the "Pad") of LolC are essential for recruiting LolA from the periplasm and priming it to receive lipoproteins. Experiments with purified LolCDE complex demonstrate that association with LolA is independent of nucleotide binding and hydrolysis, and homology models based on the MacB ABC transporter predict that LolA recruitment takes place at a periplasmic site located at least 50 Å from the inner membrane. Implications for the mechanism of lipoprotein extraction and transfer are discussed. The LolA-LolC structure provides atomic details on a key protein interaction within the Lol pathway and consti...Continue Reading

References

Sep 7, 2001·Journal of Bacteriology·N KobayashiA Yamaguchi
Jan 16, 2002·Journal of Bacteriology·Eric CascalesRoland Lloubes
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kazuhiro MasudaHajime Tokuda
Jan 31, 2003·Proteins·Simon C LovellDavid C Richardson
Jul 4, 2003·The EMBO Journal·Kazuki TakedaKunio Miki
Mar 8, 2005·Research in Microbiology·Mirium KhwajaMilton H Saier
Jul 12, 2008·The Journal of Biological Chemistry·Yuki OguchiHajime Tokuda
Jul 16, 2008·The Journal of Biological Chemistry·Shoji WatanabeHajime Tokuda
Sep 23, 2008·Journal of Bacteriology·Hiroyasu YamanakaKeinosuke Okamoto
Mar 25, 2009·Proceedings of the National Academy of Sciences of the United States of America·Suguru Okuda, Hajime Tokuda
Apr 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Juliana C Malinverni, Thomas J Silhavy
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jun 24, 2009·The Journal of Biological Chemistry·Shingo NakadaIchio Shimada
Oct 7, 2009·The Journal of Membrane Biology·Bin WangMilton H Saier
Oct 8, 2009·Bioscience, Biotechnology, and Biochemistry·Masaki YasudaShin-ichiro Narita
Nov 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Francesco ImperiPaolo Visca
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Dec 25, 2010·Cell·Athanasios TypasWaldemar Vollmer
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·T Geoff G BattyeAndrew G W Leslie
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Garib N MurshudovAlexei A Vagin
Jun 15, 2011·Annual Review of Microbiology·Suguru Okuda, Hajime Tokuda
Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Desirée C YangThomas G Bernhardt
Jun 26, 2013·Acta Crystallographica. Section D, Biological Crystallography·Philip R Evans, Garib N Murshudov
May 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Daniela MavriciTom Alber
Jul 6, 2014·Nature·Haohao DongChangjiang Dong
Feb 12, 2015·FEMS Microbiology Reviews·Nienke Buddelmeijer
Mar 4, 2015·Journal of Bacteriology·Asha S NayarSarah M McLeod
Mar 11, 2015·Journal of Bacteriology·Eric D LoVulloMartin S Pavelka
Feb 24, 2016·Nature·Yinghong GuChangjiang Dong
Jul 1, 2016·Annual Review of Microbiology·Jeremy C HendersonM Stephen Trent
Feb 22, 2017·Nature Reviews. Microbiology·Nicholas NoinajSusan K Buchanan
Apr 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Marcin Grabowicz, Thomas J Silhavy

❮ Previous
Next ❯

Citations

Oct 25, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jonathan CookAllister Crow
Dec 29, 2020·Frontiers in Microbiology·Negar Mozaheb, Marie-Paule Mingeot-Leclercq
Dec 6, 2020·The Journal of Biological Chemistry·Gregory B ColeTrevor F Moraes
Mar 6, 2021·Current Opinion in Microbiology·Jessica El RayesJean-François Collet
Mar 31, 2021·Nature Structural & Molecular Biology·Xiaodi TangHaohao Dong
Apr 5, 2021·Metabolic Engineering·Miguel C Santoscoy, Laura R Jarboe
Mar 23, 2019·EcoSal Plus·Marcin Grabowicz
Aug 5, 2021·Nature Communications·Stuti SharmaMaofu Liao

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.