Insights into function of PSI domains from structure of the Met receptor PSI domain

Biochemical and Biophysical Research Communications
Guennadi KozlovIrena Ekiel

Abstract

PSI domains are cysteine-rich modules found in extracellular fragments of hundreds of signaling proteins, including plexins, semaphorins, integrins, and attractins. Here, we report the solution structure of the PSI domain from the human Met receptor, a receptor tyrosine kinase critical for proliferation, motility, and differentiation. The structure represents a cysteine knot with short regions of secondary structure including a three-stranded antiparallel beta-sheet and two alpha-helices. All eight cysteines are involved in disulfide bonds with the pattern consistent with that for the PSI domain from Sema4D. Comparison with the Sema4D structure identifies a structurally conserved core comprising the N-terminal half of the PSI domain. Interestingly, this part links adjacent SEMA and immunoglobulin domains in the Sema4D structure, suggesting that the PSI domain serves as a wedge between propeller and immunoglobulin domains and is responsible for the correct positioning of the ligand-binding site of the receptor.

References

May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·S RongG F Vande Woude
Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
Dec 1, 1996·Journal of Biomolecular NMR·R A LaskowskiJ M Thornton
Jul 3, 1999·Trends in Biochemical Sciences·P BorkB Snel
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·O BeharC J Woolf
Apr 3, 2002·The Journal of Clinical Investigation·Alla Danilkovitch-Miagkova, Berton Zbar
May 11, 2002·Nature Reviews. Cancer·Livio Trusolino, Paolo M Comoglio
Feb 5, 2003·Journal of Magnetic Resonance·Charles D SchwietersG Marius Clore
Aug 20, 2003·Neuron·Alexander AntipenkoDimitar B Nikolov
Sep 6, 2003·Nature Structural Biology·Christopher A LoveRobert M Esnouf
Oct 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ermanno GherardiP Jonathan G Butler
Dec 20, 2003·Nature Reviews. Molecular Cell Biology·Carmen BirchmeierGeorge F Vande Woude
Nov 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt, M E Murphy
Jul 1, 1995·Journal of Biomolecular NMR·C BartelsK Wüthrich

❮ Previous
Next ❯

Citations

May 23, 2008·The Journal of Biological Chemistry·Cristina BasilicoPaolo Michieli
Dec 21, 2012·Seminars in Cell & Developmental Biology·Christian Siebold, E Yvonne Jones
Jan 19, 2011·European Journal of Cell Biology·Hartmut H Niemann
Apr 19, 2006·Journal of Molecular Biology·Sara CheekNick V Grishin
Apr 26, 2012·Expert Opinion on Therapeutic Targets·Fabiola CecchiDonald P Bottaro
Sep 30, 2014·Biochimica Et Biophysica Acta·Abhilasha PurohitRakesh K Singh
May 26, 2016·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Nele Van Der SteenChristian Rolfo
Dec 8, 2004·Current Opinion in Structural Biology·Ermanno GherardiE Yvonne Jones
Dec 15, 2015·PloS One·Birgit PiaterBjoern Hock
Dec 31, 2014·Biomedicines·Gianluca Baldanzi, Andrea Graziani
Sep 14, 2017·International Journal of Molecular Sciences·Hyunkyu ParkDo-Hyun Nam
Nov 23, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ashley C Wozniak, Judy E Anderson
Mar 17, 2010·Traffic·Dorina DotzauerNorbert Sauer
Apr 16, 2016·Oncotarget·David T ColemanJames A Cardelli
Feb 9, 2020·Oncogene·Jonas P KochMichaela Medová
May 1, 2021·Cancers·Malak SabbahGhanem E Ghanem

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Current Opinion in Structural Biology
E GherardiE Yvonne Jones
Seminars in Cell & Developmental Biology
Christian Siebold, E Yvonne Jones
© 2021 Meta ULC. All rights reserved