Insights into histidine kinase activation mechanisms from the monomeric blue light sensor EL346

Proceedings of the National Academy of Sciences of the United States of America
Igor DikiyKevin H Gardner

Abstract

Translation of environmental cues into cellular behavior is a necessary process in all forms of life. In bacteria, this process frequently involves two-component systems in which a sensor histidine kinase (HK) autophosphorylates in response to a stimulus before subsequently transferring the phosphoryl group to a response regulator that controls downstream effectors. Many details of the molecular mechanisms of HK activation are still unclear due to complications associated with the multiple signaling states of these large, multidomain proteins. To address these challenges, we combined complementary solution biophysical approaches to examine the conformational changes upon activation of a minimal, blue-light-sensing histidine kinase from Erythrobacter litoralis HTCC2594, EL346. Our data show that multiple conformations coexist in the dark state of EL346 in solution, which may explain the enzyme's residual dark-state activity. We also observe that activation involves destabilization of the helices in the dimerization and histidine phosphotransfer-like domain, where the phosphoacceptor histidine resides, and their interactions with the catalytic domain. Similar light-induced changes occur to some extent even in constitutively activ...Continue Reading

References

Sep 1, 1993·Proteins·Y BaiS W Englander
Aug 1, 1997·Journal of Magnetic Resonance·P P BorbatJ H Freed
Jan 29, 2000·Journal of Magnetic Resonance·M PannierH W Spiess
Sep 13, 2003·Science·Shannon M HarperKevin H Gardner
Dec 22, 2004·Biochemistry·Shannon M HarperKevin H Gardner
Jan 15, 2005·Journal of Magnetic Resonance·Yun-Wei ChiangJack H Freed
Sep 23, 2006·Cell·Matthew B NeiditchFrederick M Hughson
Jul 8, 2008·Nature Chemical Biology·Xiaolan YaoKevin H Gardner
Jul 7, 2009·Annual Review of Microbiology·Rong Gao, Ann M Stock
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Daniela AlbanesiAlejandro Buschiazzo
Jan 19, 2010·Nature Reviews. Drug Discovery·David A Rasko, Vanessa Sperandio
Jan 22, 2010·Journal of the American Society for Mass Spectrometry·Nian SunJohn S Klassen
Jan 17, 2012·Structure·Hedda U FerrisMurray Coles
Apr 20, 2012·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·J Valencia SMasahiro Ishiura
Jul 4, 2012·Annual Review of Microbiology·Emily J Capra, Michael T Laub
Feb 7, 2014·Nature Communications·Patricia CasinoAlberto Marina
Nov 5, 2014·ACS Chemical Biology·Andreas WinklerIlme Schlichting
Dec 4, 2014·Proceedings of the National Academy of Sciences of the United States of America·Giomar Rivera-CancelKevin H Gardner
May 20, 2015·Structure·Manasi P BhateWilliam F DeGrado
Jul 15, 2015·Molecular Microbiology·Emilio SaitaDaniela Albanesi
Jul 21, 2015·Scientific Reports·Elka R GeorgievaJack H Freed
Sep 22, 2015·Frontiers in Molecular Biosciences·Daniel NohrErik Schleicher
Oct 20, 2015·Methods in Enzymology·Gregor HageluekenOlav Schiemann
Aug 16, 2016·Journal of Molecular Biology·Christopher P ZschiedrichHendrik Szurmant
Nov 1, 2016·IEEE Access : Practical Innovations, Open Solutions·Madhur SrivastavaJack H Freed
Mar 4, 2017·The Journal of Physical Chemistry. a·Madhur SrivastavaJack H Freed
Jul 5, 2017·Journal of Bacteriology·Hrishiraj SenPeter A Lund
Jul 16, 2017·Journal of the American Society for Mass Spectrometry·Lars Konermann
Aug 12, 2017·Protein Science : a Publication of the Protein Society·Gunnar Jeschke
Nov 4, 2017·The Journal of Physical Chemistry Letters·Madhur Srivastava, Jack H Freed
Dec 28, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ivan Gushchin, Valentin Gordeliy
Apr 29, 2018·Methods : a Companion to Methods in Enzymology·Irina OganesyanDerek J Wilson
Jul 10, 2018·Chemical Reviews·Aba LosiAndreas Möglich

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Citations

Feb 26, 2020·The Journal of Biological Chemistry·Sophie BouilletRong Gao
May 28, 2019·Molecular Microbiology·Aretha FiebigSean Crosson
Mar 31, 2021·The Journal of Biological Chemistry·Jaynee E Hart, Kevin H Gardner

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