Insights into physiological roles of unique metabolites released from Plasmodium-infected RBCs and their potential as clinical biomarkers for malaria

Scientific Reports
Divya BeriG Langsley

Abstract

Plasmodium sp. are obligate intracellular parasites that derive most of their nutrients from their host meaning the metabolic circuitry of both are intricately linked. We employed untargeted, global mass spectrometry to identify metabolites present in the culture supernatants of P. falciparum-infected red blood cells synchronized at ring, trophozoite and schizont developmental stages. This revealed a temporal regulation in release of a distinct set of metabolites compared with supernatants of non-infected red blood cells. Of the distinct metabolites we identified pipecolic acid to be abundantly present in parasite lysate, infected red blood cells and infected culture supernatant. Further, we performed targeted metabolomics to quantify pipecolic acid concentrations in both the supernatants of red blood cells infected with P. falciparum, as well as in the plasma and infected RBCs of P. berghei-infected mice. Measurable and significant hyperpipecolatemia suggest that pipecolic acid has the potential to be a diagnostic marker for malaria.

References

Mar 8, 1976·Biochemical and Biophysical Research Communications·Y F Chang
Jun 1, 1983·Molecular and Biochemical Parasitology·H GinsburgZ I Cabantchik
Apr 1, 1981·Neurochemical Research·Y NomuraE Giacobini
Oct 9, 2002·Nature·Malcolm J GardnerBart Barrell
Jan 15, 2004·The Journal of Infectious Diseases·Rossarin SuwanaruskRachanee Udomsangpetch
Nov 4, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Valérie TanneurStephan M Huber
Mar 24, 2006·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Michael L Ginger
Apr 18, 2006·International Journal for Parasitology·Isabelle M Medana, Gareth D H Turner
Jul 24, 2008·Journal of Proteome Research·Jia V LiJürg Utzinger
Feb 17, 2009·Cell Host & Microbe·Kellen L OlszewskiManuel Llinás
May 9, 2012·The Journal of Infectious Diseases·Viswanathan LakshmananJohanna P Daily
May 29, 2012·Microbes and Infection·Yujin Zhang, Yang Xia
Feb 22, 2013·Transactions of the Royal Society of Tropical Medicine and Hygiene·Apurba PaulVasant Natarajan
Sep 27, 2013·Scientific Reports·Lucienne TrittenJasmina Saric
Dec 18, 2013·Metabolic Brain Disease·Giovana Reche DalazenCarlos Severo Dutra-Filho
Apr 22, 2014·PloS One·Jessica K O'HaraManuel Llinás
May 27, 2014·PloS One·Cora Lilia AlvarezPablo Julio Schwarzbaum
Sep 3, 2014·Biomedical Journal·Ghania Ramdani, Gordon Langsley
May 16, 2015·Asian Pacific Journal of Tropical Medicine·Vivek Bhakta Mathema, Kesara Na-Bangchang
Jun 26, 2015·Open Forum Infectious Diseases·Izabella SurowiecJohan Normark
Oct 27, 2015·Frontiers in Psychology·Evert BoonstraSander Nieuwenhuis
Oct 19, 2016·Molecular BioSystems·Arjun SenguptaHaripalsingh M Sonawat
Aug 31, 2016·Antimicrobial Agents and Chemotherapy·Erik L AllmanManuel Llinás
Dec 3, 2016·Nucleic Acids Research·Minoru KanehisaKanae Morishima
Mar 14, 2017·Metabolomics : Official Journal of the Metabolomic Society·Judy OrikiirizaJohan Normark
Jul 6, 2017·Nature·Liliana Mancio-SilvaMaria M Mota

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Citations

Nov 27, 2019·The Journal of Biological Chemistry·Aarti KrishnanDominique Soldati-Favre
Feb 28, 2020·Malaria Journal·Shivendra G TewariAnders Wallqvist
Jun 12, 2021·Nature Metabolism·Wael AbdrabouYoussef Idaghdour
Aug 17, 2021·Frontiers in Cellular and Infection Microbiology·Lucas Silva de OliveiraSébastien Charneau
Aug 27, 2021·Metabolites·Joachim KloehnDominique Soldati-Favre

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Methods Mentioned

BETA
glycosylation

Software Mentioned

PathwayProjector
MultiOMICS
Pathway Projector

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