Insights into Telomerase/hTERT Alternative Splicing Regulation Using Bioinformatics and Network Analysis in Cancer

Andrew T LudlowMohammed Sayed


The reactivation of telomerase in cancer cells remains incompletely understood. The catalytic component of telomerase, hTERT, is thought to be the limiting component in cancer cells for the formation of active enzymes. hTERT gene expression is regulated at several levels including chromatin, DNA methylation, transcription factors, and RNA processing events. Of these regulatory events, RNA processing has received little attention until recently. RNA processing and alternative splicing regulation have been explored to understand how hTERT is regulated in cancer cells. The cis- and trans-acting factors that regulate the alternative splicing choice of hTERT in the reverse transcriptase domain have been investigated. Further, it was discovered that the splicing factors that promote the production of full-length hTERT were also involved in cancer cell growth and survival. The goals are to review telomerase regulation via alternative splicing and the function of hTERT splicing variants and to point out how bioinformatics approaches are leading the way in elucidating the networks that regulate hTERT splicing choice and ultimately cancer growth.


Jul 1, 1992·Experimental Gerontology·Woodring E Wright, Jerry W Shay
Jan 1, 1996·Developmental Genetics·Woodring E WrightJerry W Shay
Feb 24, 2001·Neoplasia : an International Journal for Oncology Research·X YiJerry W Shay
Feb 5, 2004·Genome Biology·R Tyler HillmanSteven E Brenner
Jun 8, 2004·Molecular Biology of the Cell·Ying ZouWoodring E Wright
Jun 4, 2005·The EMBO Journal·B Kate DredgeRobert B Darnell
Feb 14, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ryan J TaftJohn S Mattick
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·C W Greider
Nov 4, 2008·Nature·Eric T WangChristopher B Burge
Jan 6, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Calin O MarianRobert M Bachoo
Apr 17, 2010·Nucleic Acids Research·Kasper D HansenSandrine Dudoit
Jan 7, 2011·Cold Spring Harbor Symposia on Quantitative Biology·T de Lange
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg
Aug 2, 2011·Mutation Research·C M BusemanJerry W Shay
Apr 9, 2013·Cell Reports·Mandy Sze WongWoodring E Wright
Nov 14, 2013·Cancer Treatment and Research·Ignacio E SchorAlberto R Kornblihtt
Dec 18, 2013·Oncogene·S Oltean, D O Bates
Mar 1, 2014·Nature Communications·Mandy Sze WongWoodring E Wright
Aug 13, 2014·Nature Reviews. Genetics·Xiang-Dong Fu, Manuel Ares
Aug 31, 2014·Trends in Genetics : TIG·Mandy Sze WongJerry W Shay
Dec 4, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A A ChiapporiJoan H Schiller
Mar 10, 2015·FEBS Letters·Semih Can AkıncılarVinay Tergaonkar
Aug 27, 2015·Oncotarget·Brody HolohanJerry W Shay
Nov 6, 2015·International Journal of Molecular Sciences·Jingna SiRongling Wu
Sep 20, 2016·The Journal of Clinical Investigation·Ekta KhattarVinay Tergaonkar
Jan 1, 2017·Genome Biology·Christelle Robert, Mick Watson
Jan 31, 2017·Nature Genetics·Floris P BarthelRoel G W Verhaak
Mar 9, 2017·International Journal of Molecular Sciences·Xuewen LiuXin Geng
Jul 11, 2017·Journal of Biotechnology·Jörg FallmannPeter F Stadler
Sep 28, 2017·Nature Reviews. Molecular Cell Biology·Yigong Shi
Mar 13, 2018·Journal of Biomedical Science·Ricardo LeãoPedro Castelo-Branco
Apr 27, 2018·Oncogene·Patricia Reyes-UribeJessie Villanueva
May 17, 2018·Methods in Molecular Biology·Anthony BayegaJiannis Ragoussis
Jun 9, 2018·Molecular Cell·Daniel DominguezChristopher B Burge
Aug 8, 2018·Nature Communications·Andrew T LudlowJerry W Shay
Sep 25, 2018·Frontiers in Molecular Biosciences·Ettaib El Marabti, Ihab Younis
Feb 15, 2019·Nature Reviews. Genetics·Jerry W Shay, Woodring E Wright


Oct 9, 2019·International Journal of Molecular Sciences·Hui-Yi LohMohd Azuraidi Osman
Oct 28, 2019·Cancers·Chad Brenner
Sep 10, 2020·Frontiers in Oncology·Chen LuoZhengming Zhu
Dec 2, 2020·Scientific Reports·Balázs VedelekImre M Boros
Oct 15, 2020·Biomolecules·Petra Procházková Schrumpfová, Jiří Fajkus
Mar 7, 2021·Cells·Yegor E YegorovAlexander N Orekhov
Apr 27, 2021·Frontiers in Genetics·Lilit NersisyanArsen Arakelyan
May 7, 2021·Mechanisms of Ageing and Development·Debasish Kumar Dey, Sun Chul Kang

Methods Mentioned

antisense oligonucleotide
interaction predictions

Related Concepts

Alternative Splicing
Gene Expression
Objective (Goal)
RNA Splicing
Transcription, Genetic
Tumor Cells, Malignant
DNA Methylation

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.