Insights into the alkaline transformation of ferricytochrome c from (1)H NMR studies in 30% acetonitrile-water

Protein Science : a Publication of the Protein Society
Sivashankar G Sivakolundu, Patricia Ann Mabrouk

Abstract

Recently, we found that ferricytochrome c (ferricyt c) undergoes significant structural changes in mixed aqueous-nonaqueous media, resulting in the formation of a mixture of alkaline-like species. The equilibrium composition of this mixture of species is dependent on the dielectric constant of the mixed solvent medium. One-dimensional (1D) and two-dimensional (2D) (1)H nuclear magnetic resonance (NMR) methods have now been used to study these alkaline-like forms in 30% acetonitrile-water solution. A native-like (M80-ligated) III* form, two lysine-ligated forms (IVa* and IVb*), and a hydroxide-ligated form (V*) were observed. Heme proton resonance assignments for these forms were accomplished using 1D (1)H NMR and 2D nuclear Overhauser effect spectroscopy methods at 20 degrees C and 35 degrees C. The chemical exchange between the alkaline forms in 30% acetonitrile solution facilitated heme proton resonance assignments. Based on examination of the heme proton chemical shifts and several highly conserved amino acid residues, the electronic structure, secondary structure, and hydrogen bond network in the vicinity of the heme in the III* form were found to be intact. Similarly, the heme electronic structure of the IVa* form was foun...Continue Reading

References

May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D J Patel, L L Canuel
Mar 28, 1978·Biochimica Et Biophysica Acta·R M Keller, K Wüthrich
Jan 10, 1989·Biochemistry·Y FengD L Di Stefano
Mar 27, 1989·FEBS Letters·X L Hong, D W Dixon
Dec 22, 1987·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J D Pettigrew, B Dreher
Jan 1, 1972·Cold Spring Harbor Symposia on Quantitative Biology·A G Redfield, R K Gupta
Feb 27, 1980·Biochemical and Biophysical Research Communications·H SennK Wüthrich
Jun 1, 1994·Nature Structural Biology·P X QiA J Wand
Aug 12, 1997·Biochemistry·L BanciP Turano
Jun 13, 1998·Biochemistry·J D CorteseC R Hackenbrock
Jul 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·B S RussellK L Bren

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Citations

Sep 23, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Adalberto Bonincontro, Gianfranco Risuleo
Apr 6, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·F Ann Walker
May 24, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Sivashankar G Sivakolundu, Patricia Ann Mabrouk
Mar 4, 2005·Chembiochem : a European Journal of Chemical Biology·Jonathan A R WorrallGerard W Canters

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