Insights into the antigenic advancement of influenza A(H3N2) viruses, 2011-2018

Scientific Reports
Patricia A JorqueraLarisa V Gubareva

Abstract

Influenza A(H3N2) viruses evade human immunity primarily by acquiring antigenic changes in the haemagglutinin (HA). HA receptor-binding features of contemporary A(H3N2) viruses hinder traditional antigenic characterization using haemagglutination inhibition and promote selection of HA mutants. Thus, alternative approaches are needed to reliably assess antigenic relatedness between circulating viruses and vaccines. We developed a high content imaging-based neutralization test (HINT) to reduce antigenic mischaracterization resulting from virus adaptation to cell culture. Ferret reference antisera were raised using clinical specimens containing viruses representing recent vaccine strains. Analysis of viruses circulating during 2011-2018 showed that gain of an N158-linked glycosylation in HA was a molecular determinant of antigenic distancing between A/Hong Kong/4801/2014-like (clade 3C.2a) and A/Texas/50/2012-like viruses (clade 3C.1), while multiple evolutionary HA F193S substitution were linked to antigenic distancing from A/Switzerland/97152963/2013-like (clade 3C.3a) and further antigenic distancing from A/Texas/50/2012-like viruses. Additionally, a few viruses carrying HA T135K and/or I192T showed reduced neutralization by A/...Continue Reading

References

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Citations

Jan 24, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Larisa V GubarevaDavid E Wentworth
May 12, 2020·Annual Review of Virology·Sigrid GoumaScott E Hensley
Feb 27, 2020·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Danuta M SkowronskiGaston De Serres
Feb 14, 2021·Antiviral Research·Mira C PatelLarisa V Gubareva
Sep 27, 2020·Journal of Virological Methods·Morgan PearsonGregg A Dean
May 30, 2021·Proceedings of the National Academy of Sciences of the United States of America·Zachary R SiaJonathan F Lovell
Jul 19, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Vivian K Y LeungPatrick C Reading

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Methods Mentioned

BETA
ELISA
glycosylation
GISAID

Software Mentioned

BLAST
Python
MEGA6
Flusurver
pandas
re
GraphPad Prism
R
GraphPad
IRMA

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