Insights into the early stage of Pinus nigra Arn. somatic embryogenesis using discovery proteomics

Journal of Proteomics
Katarína KlubicováTerezia Salaj

Abstract

The somatic embryogenesis in conifers represents a suitable model of plant regeneration system facilitating studies of fundamental aspects of an early development as well as in vitro micropropagation. The aim of our study was to deeper understand the somatic embryogenesis in the conifer tree Pinus nigra Arn. Comparative proteomic analysis based on 2D-PAGE in 1) proliferating embryogenic tissues (E) initiated from immature zygotic embryos, 2) non-embryogenic calli (NEC) initiated from cotyledons of somatic seedlings of the same genotypes, 3) embryogenic tissues that lost the maturation capacity (E-L) of two cell lines (E362, E366). Investigated pine tissues showed distinct structural features. The 24 protein spots were altered in both cell lines in comparison of embryogenic and non-embryogenic tissues. These proteins are involved in disease and defence mechanism, energy metabolism and biosynthesis of cell wall components. Two of three protein spots detected only in embryogenic form of both cell lines are similar to water deficit inducible protein LP3, the third remains uncharacterised. The loss of the maturation capacity was accompanied by changes in 35 and 38 protein spots in 362 and 366 cell lines, respectively. Only two of th...Continue Reading

References

May 1, 1996·Bioscience, Biotechnology, and Biochemistry·Y ShinozakiT Matsuzaki
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·S RavanelR Douce
Mar 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Atsushi KodanFukumi Sakai
Jan 4, 2005·Proteomics·Dustin LippertJörg Bohlmann
Oct 6, 2005·The Journal of Cell Biology·Susanne BaheErich A Nigg
Sep 18, 2007·Plant Cell Reports·Milena MarsoniCandida Vannini
Feb 27, 2008·The New Phytologist·Jorge A P PaivaChristophe Plomion
Oct 17, 2008·Nature·Jun ChengYukiko M Yamashita
Nov 1, 2008·Current Opinion in Structural Biology·Yu-Jen Chen, Masayori Inouye
Aug 26, 2009·Plant Signaling & Behavior·Jinrong WanGary Stacey
Jun 12, 2010·Annals of the New York Academy of Sciences·Susumu Ohshima, Atsushi Seyama
Nov 28, 2012·Journal of Proteomics·Alexandre Mboene NoahHans-Peter Braun
Jun 19, 2013·Journal of Proteomics·Aranzazu Gomez-GarayBeatriz Pintos
Nov 1, 2013·Proteomics·Eva Guzmán-GarcíaSebastien Christian Carpentier
Nov 4, 2015·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob

❮ Previous
Next ❯

Citations

Dec 7, 2018·PeerJ·Guadalupe Hernández-PiedraAlfonso Azpeitia-Morales
Dec 14, 2018·Frontiers in Plant Science·Victor Aguilar-Hernández, Víctor M Loyola-Vargas
Feb 15, 2019·Frontiers in Plant Science·Terézia SalajJán Salaj
Mar 7, 2019·Frontiers in Plant Science·Isabel ArrillagaEster Sales
May 15, 2020·International Journal of Molecular Sciences·Teresa Hazubska-PrzybyłEmilia Pers-Kamczyc

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.